摘要
目的提高定量检测HBV-DNA质量控制的水平。方法根据室间质评成绩,论证质量改进的必要性和可行性;对检验过程进行分析,提出误差源;结合过往室内质控数据从理论上寻找主要误差源;进行比对实验,寻找现实主要误差源及改进的操作方法;确认改进的操作方法。结果提出了4个主要的误差源,实际存在2个主要误差源;通过改进操作,室内质量控制的变异系数(CV)由改进前平均9.3%下降为3.3%,室间质评成绩也大幅提高。结论通过对实验过程的改进,定量检测HBV-DNA检验质量得到了根本性提高。
OBJECTIVE To improve the level of HBV-DNA quantity dection. METHODS The necessity and feasibility of quality control improvement on the basis of external quality assessment results were analyzed. The source of error were songht through analyzing the whole experiment process, to improv the experiment protocol. RESULTS Four main sources of error were improved, and two of them existed in reality. Through improvements in handling protocol, coefficient of variability （CV） of internal quality control has decreased to 3.3% from 9.3% before improvement in protocol. External quality control results were also increased in large-scale. CONCLUSIONS Through the improvement in experiment process, quality assurance of HBV-DNA quantity analysis has been upgraded in essence.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第16期2128-2130,共3页
Chinese Journal of Nosocomiology
关键词
室内质控
持续改进
操作细节
误差源
Quality control
Persistent improvement
Handling detail
Source of error
