摘要
目的探讨能应用于监测实时荧光定量PCR室内质控的方法。方法用Taqman实时荧光定量PCR法检测2009年3～4月的血清HBV—DNA，计算每天的阳性质控结果、标准曲线的斜率、截距和相关系数（r）及相关结果的增值（-x）、标准差（s）和变异系数（cv）。结果2009年3月份阳性质控的血清结果对数的-x、s和删分别为3．193、0．182和5．700％；斜率的-x、s和cv分别为0．274、0．015和5．470％；截距的-x、s和cv分别为12．735、0．352和2．760％；相关系数的-x、s和cv分别为0．997、0．004和0．400％。2009年4月份阳性质控的-x、s和cv分别为3．251、0．213和6．550％；斜率的-x、s和cv分别为0．274、0．016和5．840％；截距的-x、s和cv分别为12．768、0．379和2．970％；相关系数的-x、s和cv分别为0．995、0．005和0．500％。结论Taqman实时荧光定量PCR法检测HBV—DNA实验中，可同时使用阳性质控品的增值、斜率和截距作为监测手段实施室内控制，以便更好地为临床提供准确可靠的结果。
Objective To investigate better methods of indoor quality control in real - time fluorescent quan- titative PCR （QPCR）. Methods Serous HBV - DNA from March 2009 to April 2009 was detected by Taqman QPCR. Results of positive control, increment （-x） , standard deviation （ s ） and coefficient of variability （cv） of slope rate, intercept and correlation coefficient of standard curve were calculated day - by - day. Results The -x, s and cv of the resultant logarithm of positive control on March, 2009 was 3. 193, 0. 182 and 5. 700% , respectively. The x, s and cv of slope rate was 0. 274, 0. 015 and 5.470% , respectively. The -x, s and cv of intercept was 12. 735, 0. 352 and 2. 760% , respectively. The -x, s and cv of correlation coefficient was 0. 997, 0. 004 and 0. 400% , respectively. On April, 2009, the -x, s and cv of the resultant logarithm of positive control on March, 2009 was 3.251,0. 213 and 6. 550% , respectively. The -x, s and cv of slope rate was 0. 274, 0. 016 and 5. 840%, respectively. The -x, s and cv of intercept was 12. 768, 0. 379 and 2. 970% , respectively. The x, s and cv of correlation coefficient was 0. 995, 0. 005 and 0. 500%, respectively. Conclusions -x, s and cv of positive control can be used as monitoring methods in the detection of HBV - DNA by Taqman QPCR.
出处
《现代医院》
2010年第7期116-117,共2页
Modern Hospitals
关键词
聚合酶链反应
室内质控
斜率
截距
相关系数
PCR, indoor quality control, slope rate, intercept, correlation coefficient
