摘要
目的:探讨乙肝患者血清中乙肝病毒大蛋白(HBV-LP)的检测意义以及与HBV病毒复制的关系。方法:应用酶联免疫吸附试验(ELISA)和荧光定量PCR方法检测乙肝抗病毒患者血清的HBV-LP、PreS1-Ag、HBeAg及HBVDNA。结果:182份标本HBV DNA阳性,其中HBV-LP的阳性率为90.11%(164/182),高于PreS1-Ag的53.85%(98/182)和HBeAg的42.85%(78/182)(P<0.05);HBV-LP的吸光度(OD值)与HBV DNA拷贝数呈正相关,r=0.944;其OD值和DNA拷贝数随着拉米夫定治疗时间延长而下降。结论:HBV-LP能准确反映乙肝病毒的复制,与HBV DNA呈较好的正相关性;抗病毒治疗过程中HBV-LP和HBV DNA同步下降,是一个有效诊断HBV病毒复制水平的血清学检测指标。
Objective：To explore the significance of the correlation between hepatitis B virus large Protein （LP） and HBV DNA copies test. Methods：HBV LP, HBV eAg and PreS1 were measured by enzyme linked immunosorbent assay （ELISA）. Serum HBV DNA level was detected by real -time fluorescence quantitatively polymerase chain reaction （PCR）. Results：In HBV DNA positive serums, there were significant differences in positive reat in the LP, PreS1 - Ag and HBeAg, 90. 11% ,53. 85%and 42. 85% respectively, P 〈 0. 05. Serum LP level （ OD value） was closely correlated with copies of HBV DNA （ r = 0. 944）. As time going during antiviral therapy by lamivudine, HBV DNA copies and HBV - LP levels were both dropped. Conclusion： Test of HBV - LP could reflect the HBV replication correctly. There is a close correlation between the copies of HBV DNA and the level of HBV - LP. HBV DNA copies and HBV - LP level were both dropped at the same time during antiviral therapy. HBV - LP is a significant sign to moniitor HBV replication.
出处
《中国卫生检验杂志》
CAS
2009年第7期1592-1593,共2页
Chinese Journal of Health Laboratory Technology
