摘要
目的观察胃癌患者手术前后 T 细胞亚群的变化。方法采用单克隆抗体酶标法对30例胃癌患者手术前后不同时期的 T 细胞亚群即表达于所有 T 细胞的表面分子(CD3)、辅助 T 细胞的表面分子(CIM)、抑制 T 细胞的表面分子(CD8)进行动态检测。结果 (1)胃癌组 CD3、CD4阳性细胞数和 CD4/CD8比值明显低于正常对照组,CD8阳性细胞数高于正常对照组。(2)手术可引起 T 细胞亚群变化,这种变化是可逆的。(3)根治性切除术后免疫功能呈现暂时抑制到逐渐恢复的过程,但低于对照组。(4)未根治性切除术后免疫功能持续低下,CD4/CD8比值倒置,提示预后差。结论开展围手术期 T 细胞亚群动态检测,对了解胃癌患者细胞免疫状态、肿瘤是否转移和评估预后有一定的临床意义,也为胃癌患者手术后采用免疫增强治疗提供了理论依据。
Objective To observe the variety of T cell subgroup in patients with gastric carcinoma preoperatively and postoperatively.Method T cell subgroup of 30 patients with gastric carcinoma was measured dynamically by the monoclonal antibody enzyme-labelling techniques during the various period preoperatively and postopera- tively.Results (1)The number of CD3 and CD4 positive cells and CD4/CD8 rate in patients with gastric carcinoma were significantly lower than that in control group.The number of CD8 positive cells was higher than control group.(2)Operative stress may cause a change of T cell subgroup component which was convertible.(3) The immunity of patients treated by radical gastric resection that was lower than that of control group underwent a process from temporary suppression to gradual recovery.(4)The immunty of patients treated by palliative gas- tric resection was constantly depressed and CD4/CD8 rate was reversed.It was suggested that the prognosis was poor.Conclusion Perioperative T cell subgroup surveillance was important to understand the cellular immuni- ty,predict metastasis and prognosis in patients with gastric carcinoma.
出处
《中华普通外科杂志》
CSCD
1998年第4期216-218,共3页
Chinese Journal of General Surgery
关键词
胃肿瘤
T细胞亚群
手术前
手术后
Stomach neoplasm
Allergy and immunology
T-lymphocyte subsets
