摘要
应用基因芯片技术,从基因水平分析隐丹参酮对胰岛素抵抗卵巢颗粒细胞基因表达的影响。采用猪卵巢颗粒细胞作为体外研究对象,利用磷酯酰肌醇-3激酶(PI-3K)特异性抑制剂——沃曼青霉素人工诱导猪卵巢胰岛素抵抗的细胞模型,以0.02g/L隐丹参酮进行干预,继续培养48h后,以Trizol法提取总RNA,采用猪全基因表达谱芯片技术筛选出差异表达基因。隐丹参酮组与沃曼处理组相比,共筛查出42个差异基因,其中22个基因表达下调,20个基因表达上调。研究表明,隐丹参酮可以通过调节多个基因、作用多个信号途径来改善胰岛素抵抗卵巢颗粒细胞的状态。
The mechanism of Cryptotanshinone in treating ovary insulin resistance is identified in this paper. The ovarian granulose cells from porcine follicles were isolated and cuhured in vitro, and insulin resistance was induced by treatment with Wortmannin. The cells were divided into 4 groups, the blank control group cultured with DMSO, the Wortmannin group cultured with Wortmannin 250 nmol/L, the Cryptotanshinone group cultured with Wotrmannin 250 nmoYL as well as 0.02 g/L Cryptotanshinone. After being incubated for 48 h, the total RNA was isolated from cells, and the differently expressed genes were screened from them by the gene chip technique. Then they were analyzed by molecule annotation system to get differential gene expression profiles and pathways of the four groups. The results show that 42 differential genes are screened through the channel gene chip technique, including 22 down- regulation genes and 20 up-regulation genes. These differential expression genes have various biological functions, such as lipid metabolism, intercellular signal transmission and so on. And the Cryptotanshinone may improve ovary insulin resistance by adjusting many genes and signal pathway. It is implied that Cryptotanshinone may be used to treat endocrine disease and reproductive barrier of polycystic ovary syndrome.
出处
《科技导报》
CAS
CSCD
北大核心
2009年第15期39-43,共5页
Science & Technology Review
基金
黑龙江省自然科学基金重点项目(ZJY0606-01)
关键词
基因芯片
隐丹参酮
胰岛素抵抗
卵巢颗粒细胞
DNA microarray
Cryptotanshinone
insulin resistance
ovarian granulosa cells
