摘要
目的应用基因芯片研究三氧化二砷（As2O3）处理前后K562细胞基因表达的变化。方法提取As2O3处理前后K562细胞的总RNA,纯化为mRNA后再反转录为cDNA。cDNA经限制性内切酶Sau3AI切割后,cDNA片段分别用Cy3和Cy5标记,与自制的包含348个基因片段的胎盘库芯片杂交。结果杂交结果经扫描和软件分析,发现了11个差异表达的基因片段,其中有3个基因片段与细胞凋亡密切相关。结论 我们构建的胎盘库基因芯片可以成功地用于研究药物作用前后基因表达的变化。
Objective To investigate differential gene expression in apoptotic cells induced by As2O3, and identify novel apop-tosis-related genes. Method Apoptosis of K562 cells cultured in RPMI 1640 medium supplemented with 10% calf serum was induced by As2O3. Total RNA of the apoptotic and normal cells were then extracted, purified and subject to reverse transcription into firt-strand cDNA, labeled with Cy3/Cy5. Placenta DNA microarrays containing 348 DNA fragments were used to analyze the changes in gene expressions in the cells treated with As2O3. Result Eleven differentially expressed genes were i-dentified in the apoptotic cells in comparison with the normal cells, 3 of which were associated with apoptosis, while the others were related to cell growth and proliferation. Conclusion The placenta DNA microarrays we constructed may well apply to the analysis of the differentially expressed genes.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第9期772-775,共4页
Journal of First Military Medical University
基金
广州市重大科技攻关项目(99Z02201)
广东省自然科学基金(984092)
