摘要
建立鳗鱼中15种磺胺、3种喹诺酮类以及4种四环素族抗生素残留的超高效液相色谱-串联质谱同时测定方法。采用酸性EDTA-Mcllvaine缓冲液提取,经HLB固相萃取小柱富积、净化后进行UPLC分离,电喷雾串联质谱进行检测,多离子反应监测(MRM)模式定量。添加水平为5、10、20μg/kg时各种化合物的平均回收率范围为72.4%～97.3%,RSD值均小于14.4%。该法简单、快速、灵敏,适用于鳗鱼中多磺胺组分、喹诺酮及四环素族组分残留的同时测定。
A robust and sensitive UPLC-MS-MS method was developed for the simultaneous determination of residues of 15 sulfonamides, 3 fluoroqninolones and 4 tetracylines in eel. The target compounds were extracted with acidic EDTA-Mcllvaine buffer solution, and enriched and cleared up using a hydrophilic-lipophilic balance （I-ILB） SPE cartridge. After seperation on UPLC BEH C18, the analytes were detected using a tandem mass spectrometry and quantified in multiple reaction monitoring （MRM） mode. At the spike levels of 5, 10 and 20 μg/kg respectively, the average recoveries for all the compounds were 72.4%-97.3%, and the variation coefficients were all under 14.4%. This method is simple, fast, sensitive, and particular for the simulaneous determination of sulfonamides, fluoroquinolones and tetracylines in eel.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第14期167-170,共4页
Food Science
基金
国家质量监督检验检疫总局科技计划项目(2007IK147)
福建省科技计划重点项目(2007Y001)
