摘要
建立了HPLC法同时分离并检测鱼、虾等水产品中14种磺胺类抗菌药物。样品经无水硫酸钠脱水后 ,乙腈提取药物 ,再用乙腈饱和正己烷脱脂净化。以乙腈 -3 %冰醋酸为流动相 ,采用梯度淋洗 ,于268nm波长处检测。该法的检出限为0.01～0.02mg/kg ,样品的加标回收率为66 %～92%。
A method for the simultaneous determination of14sulfonamides residues in aquatic products by HPLC was established.Sulfonamides in samples were first extracted with acetonitrile after dehydration with anhydrous sodium sulfate,then purified fromfats by extraction with acetonitrile_saturated n_hexane,and finally determined by HPLC with a UV detector at a wavelength of268nm.Gradient elution with a mobile phase of3%acetic acid-acetonitrile was employed at a flowrate of1.0mL/min.The detection limits were found to be0.01～0.02mg/kg,and recoveries of66%～92%were obtained.
出处
《分析测试学报》
CAS
CSCD
北大核心
2004年第5期122-124,共3页
Journal of Instrumental Analysis
基金
973课题 (2002CB412402)
中国科学院重大项目 (KJCX315W -215)
