摘要
采用静态试验法,将鲫鱼暴露于4、16、64mg·L-1洛克沙胂中48、96、144h后,检测了鱼鳃、肾脏和肝的Na+-K+-ATP酶活性。结果表明,在同一组织中,随着处理浓度的提高,洛克沙胂对鲫鱼各组织Na+-K+-ATP酶活性抑制作用增强。对于不同组织,同一处理浓度的洛克沙胂对鲫鱼鳃中Na+-K+-ATP酶活性抑制作用更为明显,其次是肝和肾(P<0.01)。在同一浓度下,洛克沙胂对鲫鱼各组织Na+-K+-ATP酶活性抑制率呈明显的时间-效应关系,暴露时间越长,抑制率越高(P<0.01)。此外,单细胞凝胶电泳试验(SCGE)结果表明,鲫鱼暴露于0.5、1、2mg·L-1洛克沙胂中72h能引起鲫鱼肾细胞明显的DNA损伤,并在设定剂量范围内呈现一定的剂量-效应关系;同时,鲫鱼肾细胞于体外暴露于1、10、100、500、1000mg·L-1洛克沙胂3、6、12h后,可引起严重的DNA损伤。
Roxarsone is a veterinary feed additive with a long history of use as a growth promoter in stock raising. Although this drug has been widely used for its low price and convenient usage, roxarsone is actually still under evaluation because of the uncertainty regarding the mechanism involved in the biochemical toxicity and genotoxicity to aquatic organisms.In this paper, experiments were conducted to study the effects of roxarsone on the Na^＋-K^＋-ATPase activity in tissue（ gill, kidney and liver）and DNA damage in kidney cell of crucian carp（Carassius auratus ）. Firstly, all fishs were divided into 4 testing groups and were exposured to solutions with different concentrations of roxarsone（0, 4, 16, 64 mg· L^-1）respectively.Gill, kidney and liver of crucian craps were collected for determination of Na^＋-K^＋-ATPase activity at 48, 96 and 144 hours after exposure. The result showed that Na^＋-K^＋-ATPase activity of three tissues were significantly inhibited by roxarsone. The degree of sensibility of Na^＋-K^＋-ATPase to roxarsone was expressed as following：gill〉liver〉kidney. In addition, Na^＋-K^＋-ATPase activity decreased gradually as roxarsone doses and exposure time were raised, which showed dose-effect relationship and time-effect relationship.Secondly, the single cell gel electrophoresis test（ SCGE, comet assay ）of crucian carp kidney cells in vivo and vitro were conducted to evaluate the toxicological effects of roxarsone on typical aquatic species. The results of the SCGE showed that roxarsone at the dose of 0.5, 1, 2 mg·L^-1 could induce DNA damage in crurian kidney cells in vivo. The damage caused by roxarsone were obvious.The extent of DNA damage increased gradually as roxarsone doses were raised, which showed dose-effect relationship. At the same time, roxarsone at the dose of 1, 10, 100, 500, 1 000 mg·L^-1 could induce DNA damage in crucian crap kidney cells at 3, 6 and 12 hours after exposure in vitro. The action of DNA damage showed a dose-effect relationship and time-effect relationship. The above results showed roxarsone might have potential biochemical toxicity and genotoxicity to aquatic organisms.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2009年第7期1374-1378,共5页
Journal of Agro-Environment Science
基金
中国博士后科学基金项目
江苏省博士后科研资助项目
