摘要
目的:建立HPLC-ELSD法测定吉祥草中皂苷A含量的方法。方法:采用Aglient Eclipse-XDB-C18色谱柱(4.6 mm×150 mm,5μm),甲醇-水(46∶54)为流动相,流速1.0 mL.min-1,柱温40℃,ELSD漂移管温度40℃,雾化空气压力0.30 MPa。结果:皂苷A进样量在0.618～9.888μg呈良好线性关系(r=0.999 8),平均回收率为99.11%,RSD 2.47%。结论:该方法简便、快速、准确、灵敏度高,可用于吉祥草中皂苷A含量测定。
Objective： To establish HPLC-ELSD method for the determination of saponin A in Reineckia carnea. Method： The column was Elipse-XDB-Cls （4.6 mm × 150 mm, 5 μm） and mixture of methanol-water （46： 54） as mobile phase; the flow rate was 1.0 mL · min-1 and the column temperature was at 40 ℃. Drift tube temperature was 40 ℃ , pressure of nebulizer gas was 0.3 MPa. Result： The linearity of saponin A was good in the range of 0. 618-9. 888 μg （r =0. 999 8）. The average recovery was 99. 11% , with RSD of 2.47%. Conclusion： The method is convenient, rapid, accurate with high sensitivity and can be used reliably for analyzing saponin A in R. carnea.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期112-114,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省中药现代化项目(黔科合[2007]5016)
贵州省中药现代化项目(黔科合[2011]5081)
贵州省科技厅科技计划项目(黔科合[2009]4001)
贵阳市科技计划项目(筑科合[2009]9304)
贵州省科技创新人才团队项目
