摘要
目的:探讨补充3周丙酮酸钙对于小鼠力竭后组织损伤的保护作用机制。方法:雄性小鼠40只,随机分为安静对照组(C组)、运动对照组(Ec组)、丙酮酸钙组(P组)、运动丙酮酸钙组(Ep组)各10只,C及Ec组小鼠每天灌服10%阿拉伯胶溶液,P及Ep组每天给予600mg/kg的丙酮酸钙灌胃,共3周。3周末时各组大鼠均进行力竭运动实验,检测肝脏组织中过氧化氢(H2O2)和谷胱甘肽(GSH)的含量、ATP酶活力。结果:与Ec组比较,Ep组小鼠运动至力竭时间明显延长,运动能力显著提高(106.9±24.7min、167.0±31.2min,P<0.01)。Ep组GSH含量明显高于其它各组(P<0.05)。运动力竭后Ec组和Ep组H2O2含量明显高于C、P组,Ep组低于Ec组(P<0.01)。结论:补充丙酮酸钙能明显提高小鼠力竭运动能力和抗氧化损伤能力,其可能原因是提高GSH抗氧化能力及抵抗部分ATP酶的失活。
Objective： To investigate the effects of calcium pyruvate supplementation for 3 weeks on oxidation resistance after exhaustive exercise in mice. Methods： Forty female mice were randomly divided into 4 groups： control group （C group）, exercise control group （Ec group）, calcium pyruvate group （P group） and exercise-calcium pyruvate group （Ep group）. The mice in pyru- vate-treated groups received calcium pyruvate, and those in control groups received placebo （water） 600 mg/kg body weight every day for 3 weeks. Exercise ability, GSH, H2O2 and ATPase were measured after the experiment. Results： At the end of the experi- ment, there was a statistically significant improvement of exercise ability in Ep group as compared with C group （106.85±24.66 min vs 166. 95±31.18 rain, P〈0. 001）. A statistically significant increase of GSH in Ep group was observed as compared with other groups （P〈0 01）. There was a significant decrease in H2O2 in Ec and Ep groups as compared with control groups at the end of ex- haustive exercise, and the increase of ATPase in Ep group was significantly different from that in Ec group （P〈0. 05）. Conclusion： Supplementation of calcium pyruvate for 3 weeks can obviously improve the exercise ability and anti-oxidation capacity in mice by elevating the ability of GSH oxidation resistance system and partially antagonizing the deactivation of ATPase.
出处
《中国康复》
2009年第3期147-148,共2页
Chinese Journal of Rehabilitation
基金
大连医科大学大学生科技创新活动基金
基础医学院大学生科研项目共同资助
