摘要
用ESR技术研究发现,不同类型的肌纤维在运动和安静时,自由基含量均明显不同。安静时,有氧能力高的红肌自由基含量高,运动后白肌纤维的自由基明显增加,井出现一个文献中从未报导过的新的自由基信号(g=2.001,width=0.2mT),该信号与疲劳程度相关。红肌运动后自由基变化不明显,从而找到了运动时不同肌纤维自由基代谢差异性的最直接的证据。
Whether the free radical(FR) is one of the factors to induce the difference in fatigability anddamage of different fiber types has been discussed in the field of exercise physiology recently. In ourresearch, 20 trained male S-D rats were randomly divided into two groups and were killed at rest (onegroup) or after 45m/min running to fatigue (the other group). White quadriceps (WQ), mixed quadriceps(MQ)and red quadriceps(RQ)intact samples were monitored with electron spin resonance (ESR) at 100K. Results: 1. Rest group: only one small radical signal 'Q' (g =2.004) was detected in WQ, two large FRsignals 'Q', 'T' (g = 2.004, 1.937) and one small signal (g = 2.026) were detected in RQ. The MQ hadintermadiate signal between WQ and RQ. 2. Fatigue group: The FR of WQ increased a lot, the FR of RQhad no significant change. The FR of MQ increased between WQ and RQ. A new sharp major FR signal(g=2.001, width 0.2mT) was also found in all of the fatigue white skeletal muscles. This new radical hasnever been reported before in vivo. The pattern of this FR is similar to the superoxide which has beenreported in vitro. Conclusion: 1. FR levels of different muscle fiber tvpes are different. The higher oxida-tive capacity the fiber has, the higher level of FR. 2. After intensive running to fatigue only FR of WQsignificantly increased, perhaps this is one of the factors of difference in fatigability and damage ratesof the various skeletal fiber types.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
1991年第3期135-139,共5页
Chinese Journal of Sports Medicine
关键词
ESR研究
疲劳
肌纤维
自由基
eletctron spin resonance
free radical
different fiber types
muscle fatigue
