摘要
目的探讨干细胞因子（SCF）在狼疮肾炎（LN）患者肾组织中的表达及其与狼疮活动、肾损害有关指标的关系。方法采用原位杂交和免疫组织化学技术，分别在基因和蛋白质水平检测[34例LN，16例慢性肾小球肾炎（CGN），8名健康对照]肾组织中SCF及其受体c—kit（CD117）的表达，同时检测肥大细胞、臣噬细胞及α-平滑肌肌动蛋白（α—SMA）阳性细胞的浸润情况。计算狼疮肾组织活动指数和慢性指数得分及肾问质损害程度。结果①LN及CGN。肾组织中SCF的表达及肥大细胞较对照组明显增加（t=6．03～14．25，P〈0．01）。间质损害程度相似的LN与CGN相比较，肾间质SCF、肥大细胞差异无统计学意义。②SCF与肥大细胞、巨噬细胞、α-SMA及临床指标的相关性：相应部位SCF的表达、α-SMA的表达、肥大细胞、巨噬细胞的浸润数量呈显著正相关（r=0．47～0．86，P〈0．01）。SCF的表达、d—SMA的表达及巨噬细胞数量与肾病理活动性指数、慢性化指数、肾间质损害程度、尿蛋白定量均呈显著正相关（r=0．34～0．93，P〈0．05或0．011，与肌酐清除率（Ccr）呈明显负相关（r=0．39—0．61，P〈0．01）。SCF及巨噬细胞与抗dsDNA滴度、补体C3、SLE疾病活动指数（SLEDAl）积分也有显著相关性。肥大细胞数与LN慢性指数特别是肾间质损害程度呈明显正相关（r=0．86，r=0．93，P〈0．01）与CPT呈明显负相关（r=-0．56，P〈0．01）。与LN活动指标无相关性。结论SCF在LN肾组织呈广泛表达，其表达量与多种炎症细胞、肾脏同有细胞、肾功能、尿蛋白、疾病活动性及严重程度等密切相天，提示SCF可能是LN病理过程中一个关键的全程参与者．
Objective To investigate the renal expression of stern cell factor （SCF） in lupus nephritis （LN） and its correlation with disease activity and renal injury parameters. Methods Histochemical stain was used to examine all renal specimens （LN group n=34, chronic glomerulonephritis n=16, control group n=8）. Hybridization in situ and inmumohistochemistry were used to detect the expression of SCF and infiltration of mast cells, macrophages , α-SMA （＋） cells in renal tissues of the two groups. SPSS software was used for correlation analysis. Results （1） The expressions of SCF and mast cells were scarce or absent in the renal tissue of the control group. HoweveR＇, they increased markedly in lupus nephritis and CGN （t=6.03-14.25, P〈 0.01）. But there was no significant difference between LN and CGN in SCF and mast cells in renal interstitium. （2） Correlation of the expression nf SCF and α-SMA, mast cells and macrophages with clinical parameters. Positive correlation was observed among the expression of SCF and α-SMA and the number of mast cells and maerophages （r=0.47-0.84, P〈0.01 ） at their corresponding locations. The expression of SCF and α-SMA and the number of macrophages were positively correlated with renal pathological active index, chronic index, albuminuria and the injury, of renal interstitium （r=0.34-0.93, P〈0.05 or 0.01 ）; meanwhile, it was negatively correlated with Ccr（r=-0.39--0.61, P〈0.01 ）. There was significant correlation between SCF, macrophages and anti-dsDNA antibody, complement C3 level, SLE disease activity index （SLEDAI）. The number of mast cells in renal interstitium was positively correlated with chronic indexes and the injury of renal interstitium （r= 0.86, r=0.93, P〈0.01 ） and negatively correlated with Ccr （r=-0.56, P〈0.01 ）, but not correlated with active index and albuminuria （r=0.27, r=0.23,/9〉0.05）. Conclusion The expression of SCF is widespread in kidney,and it is markedly correlated with various kinds of inflammatory cells, renal inherent cells, renal function, and urine protein levels. SCF may be an critical participant in the initiation and progression of renal injuries in human lupus nephritis.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2009年第6期376-380,I0001,共6页
Chinese Journal of Rheumatology
基金
山东省卫生厅科学基金（2005HZ022）
