摘要
研究了溶氧对Brevibacterium lactofermentation分批发酵生产L-异亮氨酸(Ile)的影响,提出了前10h恒700r/min以维持溶氧在35%以上,10h后调至600r/min以维持溶氧在15%～20%的两阶段供氧控制模式。与对照相比,获得了较高的产率(0.094g/g)和糖耗速度(4.76g/L·h),在较短时间内(52h)获得较高的Ile产量(23.3g/L),比结果最好的单一搅拌转速(600r/min)提高11.6%。生产强度(0.448g/L·h)比恒定搅拌转速(500、600、700、800r/min)控制下的过程分别提高了83.6%、28.7%、44.9%、35.7%。最后采用代谢通量分析对该结果产生的原因进行了定量解释。
The effect of dissolved oxygen （DO） on L-isoleucine production by Brevibacterium lactofermentation was investigated in batch fermentation process. A two-stage DO control strategy was developed, in which the agitation speed was controlled at 700 r/min during in the first 10 h to achieve a DO level up to 35%, and then switched to 600 r/min after 10 h to maintain a DO level at 15% --20 %. A relative high yield （0. 094 g/g） and glucose consumption rate （4.76 g/L·h） were obtained. The high isoleueine production （23.3 g/L） was achieved in a relative short period （52 h）, and it was increased by 11.6o,6 compared to the highest value ofby using single agitation speed （600 r/min）. According to the results in constant agitation speed （500, 600, 700, 800 r/min）, the productivity （0. 448 g/L·h） was increased by 83.6 %, 28.7%, 44.9 %, and 35.7 %, respectively. Finally, the reason for the increase of isoleucine production with the proposed two-stage DO control strategy was quantitatively described in detail by analysis of metabolic flux distribution in metabolic network of isoleucine production by Brevibacterium lactofermentation.
出处
《工业微生物》
CAS
CSCD
2009年第3期11-16,共6页
Industrial Microbiology
基金
国家高技术研究发展计划(863)项目资助(No.2007AA02Z229)。
