摘要
①目的调查青岛市区学龄前儿童巨细胞病毒感染情况。②方法用抗人巨细胞病毒（ＨＣＭＶ）早期抗原（ＥＡ）的单克隆抗体（ＭｃＡｂ），通过间接免疫过氧化物酶法，检测尿标本细胞培养中的ＨＣＭＶ－ＥＡ．③结果１９５名入托儿童尿标本中，有８９名（４５．６％）ＨＣＭＶ－ＥＡ检测阳性，其中１岁以下婴幼儿排毒率最低（１７．４％），１～２岁儿童排毒率最高（６５．６％）。９１名未入托的学龄前儿童中，有２１名（２３．１％）检测到ＨＣＭＶ，与幼儿园儿童排毒率（４５．６％）相比显著有差异性（χ２＝１３．３４７，Ｐ＜０．０１）。④结论青岛市区儿童主要在１～２岁感染ＨＣＭＶ，幼儿园内ＨＣＭＶ的传播流行主要由儿童间密切接触感染所致，这些排毒儿童对血清ＨＣＭＶ抗体阴性的育龄期妇女有较大危害。
Objective To investigate the infective situation with human cytomegalovirus (HCMV) in preschool children in Qingdao. Methods The early antigens of HCMV( HCMV EA) were determined by corresponding monoclonal antibody (McAb) using indirect immnoperoxidase assay. Results 89 out of 195 urine specimens of children in nursery were positive for HCMV EA. The lowest positive rate(17.4%) appeared in infants under 1 year. The highest positive rate(65.6%) appeared in children between 1 and 2 years old. The positive rate of HCMV EA infection in 91 preschool children away from nursery was 23.1%(21/91), which was significantly lower than that of preschool children in nursery( χ 2= 13.347, P< 0. 01). Conclusion Children between 1 and 2 years old in Qingdao area were susceptible to HCMV infection. The contact infection from children to children was the main spread pattern of HCMV infection in nursery. Chilrden with positive HCMV EA infection were dangerous to the child bearing age nurses whose sera were negative for HCMV antibody. So they may be the source of congenital HCMV infection.
出处
《青岛医学院学报》
1998年第2期97-99,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省卫生厅青年基金
关键词
巨细胞病毒感染
儿童
学龄前
流行病学
cytomegalovirus infection
child, preschool
epidemiologic methods
