摘要
目的：建立检测下呼吸道感染婴幼儿粘膜细胞呼吸道合胞病毒（RSV）抗原的方法。方法：以生物素标记兔抗RSV抗体，鼻咽部粘膜细胞涂片与该抗体作用后加链霉亲和素-过氧化物酶反应，二氨基联苯胺显色，显微镜下观察结果。结果：RSV抗原总阳性率为41．6％，临床典型和可疑病例抗原检出率分别为47．9％和44．9％，临床不典型者阳性率12．5％（X2=7．9，P＜0．05）。阳性标本中细胞着成棕色，易与阴性标本相区别。结论：本法简便、快速，具有一定敏感性和特异性，适合于基层推广应用。
ObjectiveTo establish a method for detecting the respiratory syncytial virus(RSV) infection in theexfoliated epithelial cells of lower respiratory tract in infants and young children. Methods:The rabbit antibodyagainst RSV was labelled with biotin; the streptavidin-Peroxidase and diaminobenzidine were added to cover thesmear of exfoliated respiratory epithelial cells after the reaction of the smear with the biotin-laballed antibody ; the results were examined using a light microscope. Results: The total RSV antigen positive rate was41. 6%; the positive rates in the clinically typical and suspected cases were 47. 9% and 44. 9% respectively ; thepositive rate in the clinically atypical cases was 12. 5 % (X2= 7. 9,p<0. 05). The typical staining pattern exhibited brown color and the positive cells were easy to be differentiated from the negative cells. Conclusion: Themethod is simple and fast with afairly high sensitivity and certain specificity, and especially suitable to be used inthe hospitals at the primary level.
基金
浙江省卫生厅医学科技研究基金
关键词
生物素
链霉亲和素
过氧化物酶法
呼吸道
RSV
Biotin-streptavidin-peroxidase method
Respiratory syncytial viruses
Paramyxovirus infection /diag
Immunoenzyme techniques
