摘要
用抗人巨细胞病毒(HCMV)早期抗原的单克隆抗体,通过间接免疫荧光技术和间接免疫酶染色,对细胞培养中的CMV早期抗原(EA)进行检测。检测结果,132份临床标本中有43份常规细胞培养(CCC)出现HCMV特征性细胞病变(CPE),其中40份在接种后24h早期抗原检测(DEA)阳性,敏感性达93%;其余89份CCC阴性,有5份DEA阳性,特异性为94.4%.同时用HCMVAD169进行的试验结果表明,HCMV感染细胞24h即可在接种10^(-5)病毒稀释度的细胞培养瓶中检测到CMV-EA,而同一稀释度CCC则于21d才能观察到CPE出现。提示DEA不仅敏感特异,而且与CCC相比,其最大的优点是快速,可在接种标本后24h甚至更短的时间内完成,CCC则平均需要16d。
Monoclonal antibodies, which are reactive to cytomegalovirus (CMV) early anti-
gens, were used in an indirect immunofluorescence technique (IIF) and an indirect
mmunoperoxidase (IPA) staining procedure for rapid detection of cytomegalovirus
in cell culture. The whole staining was in the shell vial. Of 132 clinical spe-
imen tests, 43 produced specific CMV cytopathic effect (CPE) in conventional cell
eulture (CCC) and of these 43, 40 were positive by the method of the detection of
carly antigens (DEA) 24h after inoculation (sensitivity, 93%). Of the remaining 89
clinical specimens negative on CCC tests, 5 were positive by the DEA method (spe-
ificity, 94.4% ). Simultaneously, our experiment with HCMVAD 169 showed: DEA
procedure at 24h after infection detected CMV early antigens in a 10^(-5) dilution of
the AD169 strain in cell culture. but CMV-CPE was not observed in the same
dilution until day 21 postinfection. In summary, the DEA test was both sensitive and
specific. It has several advantages over the CCC system, the most important of which
is the rapidity with which it can achieve a diagnosis 24h after infection or
even earlier, whereas a diagnosis by the CCC method took 16 days. There is no prac-
tical reason why this simple and rapid method should not be used to examine HCMV
antigens in clinical specimens in any virology laboratory where call culture ca be
made.
出处
《青岛医学院学报》
1991年第3期197-201,共5页
Acta Academiae Medicinae Qingdao Universitatis
关键词
巨细胞病毒
单克隆抗体
病毒
检测
cytomegaloviruses
men
antibodies, monoclonal
antigens, viral
cytological technics
