摘要
从山东省山东大学南部山区土壤中分离得到1株具有广谱抗真菌活性的链霉菌SD-07菌株。根据形态特征、生理生化反应和16S rDNA序列分析结果,链霉菌SD-07鉴为定为纺锤链霉菌(Streptomyces netropsis)。分离纯化了SD-07产生的抗真菌活性成分并获得其结晶CA-SD07。抗真菌活性检测结果发现:CA-SD07对酵母菌、担子菌、接合菌等多种真菌具有广谱抗菌作用。对CA-SD07进行性质研究发现,抗真菌活性成分CA-SD07抗菌谱广且抗菌活性高(约等于2倍的两性霉素B),是戊烯和七烯混合的多烯大环内酯类抗真菌抗生素。
Streptomyces sp. SD-07 was isolated from the soil of a mountain area in the south of Jinan, Shandong Province. This strain exhibited broad-spectrum and high antifungal activity. Based on the results of prehminary morphological, cultural, physiological and biochemical studies on this strain, and its 16S rDNA sequences, strain SD-07 was classified as Streptomyces netropsis. The antifungal compound CA-SD07 were extracted from the mycelia and then crystallized, and named as CA-SD07. CA-SD07 could inhibit many fungi such as Ascomycetes, Basidiomycetes and Zygomycetes. The data from ultraviolet-visible absorption and high-performance liquid chromatography / diode array detection （HPLC / DAD） indicated that CA-SD07 was a mixture of pentaene macmlide and heptaene macrolide antibiotics, and the major compound was pentaene macrolide. CA-SD07 has wider antifungal spectrum and higher antifungal activity（ about two times Amphotefic B）.
出处
《山东大学学报（理学版）》
CAS
CSCD
北大核心
2009年第5期28-32,共5页
Journal of Shandong University(Natural Science)
基金
山东省自然科学基金资助项目(Y2008D15)
关键词
纺锤链霉菌
抗真菌抗生素
多烯大环内酯抗生素
Streptomyces netropsis
antifungal antibiotics
pentaene macrolide antibiotics
