摘要
在离体条件下及棉花植株内测试了匐柄霉对大丽轮枝菌的抑制作用。结果表明，在离体条件下，匐柄霉菌的菌丝体或其培养滤液，对大丽轮枝菌的生长和微菌核形成均有影响。将形态发生变异，不能形成微菌核的大丽轮枝菌菌株转移至ＰＤＡ培养基后，其微菌核形成能力不再恢复。匐柄霉培养滤液的抑菌物质受热不稳定，１００℃处理１０分钟后活性丧失。抑菌物质经硫酸铵、乙醇沉淀初步测定，属非蛋白次生代谢产物。匐柄霉经接种可进入棉株体内，定殖率为７５％～１００％，分布于棉苗根、茎的维管束组织中。与轮枝菌先后或同时混合接种，匐柄霉可减轻棉花黄萎病的症状，以棉苗栽种前用匐柄霉菌分生孢子悬浮液沾根处理效果最明显。
Inhibiting effect of Stemphylium sp. on Verticillium dahliae was tested both in vitro and in vivo. The result indicated that mycelial growth and microsclertium formation of V.dahliae were inhibited by either mycelial or cultural filtrate of Stemphylium sp.. Mycelial type of V.dahliae which did not produce microsclerotia could produce. They were not reversed to wild type after 2-3 times mass transfers. The microbe seemed to be one of the environmental factors affecting microsclerotium formation of V. dahliae. The inhibiting factor of Stemphylium sp. in cultural filtrate was not heat stable and lost activity at 100℃ treatment for 10min. It was identified as a nonprotein secondary metabolite by using (NH4)2SO4 and alcohol precipitation method. The propagating rate of Stemphylium sp. inside cotton stem was 75%-100% after inoculation. The disease index of cotton wilt was reduced by Stemphylium sp. inoculation before and after V.dahliae inoculation.
出处
《中国生物防治》
CSCD
1998年第1期14-17,共4页
Chinese Journal of Biological Control
基金
江苏省"九五"攻关项目
新疆农业大学校长基金
关键词
匐柄霉菌
大丽轮枝菌
微菌核
抑制作用
棉花
Stemphylium sp. Verticillium dahliae cotton vascular bundlemicrosclerotium inhibition
