摘要
目的探讨ＹＮＺ２２位点串联重复序列（ＶＮＴＲ）的杂合性丢失在原发肝癌发生发展中的作用．方法应用多聚酶链反应（ＰＣＲ）技术扩增３５例原发肝癌和正常肝组织ＹＮＺ２２位点的ＶＮＴＲ区，对原发肝癌的杂合缺失进行分析．结果原发肝癌３５例，属信息个体２５例，占７１４％．信息个体中２５例检出ＹＮＺ２２位点杂合缺失（ＬＯＨ）１２例，杂合缺失率为４８０％．分析ＬＯＨ与临床病理参数间的关系发现，原发肝癌ＹＮＺ２２位点杂合缺失与血清ＨＢｓＡｇ和ＡＦＰ水平、组织学分级、肿瘤大小及远处转移无关．结论ＹＮＺ２２位点杂合缺失是原发肝癌的常见改变，可能参与了原发肝癌的发生与发展．
IM To evaluate the role of heterozygosity (LOH) loss at YNZ22 locus in the development of hepatocellular carcinoma.METHODS Polymorphic analysis of YNZ22 locus that contains a variable number of tandem repeat (VNTR) was performed in 35 surgically resected specimens of hepatocellular carcinomas and adjacent normal tissues by the polymerase chain reaction (PCR).RESULTS Constitutional heterozygosity (informative) was found in 25/35 (714%) of hepatocellular carcinomas. Loss of heterozygosity (LOH) at YNZ22 locus was detected in 480% of informative cases. No significant difference existed between LOH at YNZ22 and serum levels of HBsAg and AFP,histological grades, tumor size and distant metastasis (P>005).CONCLUSION LOH at YNZ22 locus is a common change in hepatocellular carcinomas and may be involved in carcinogenesis of hepatocellular cancer.
关键词
肝肿瘤
遗传学
染色体
染色体缺失
VNTR
PCR
liver neoplasms/genetics
chromosomes, human, pair 17
minisatellite repeats
heterzygote
chromosome deletion
