摘要
应用多聚酶链反应（ＰＣＲ）和Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交技术，检测５０例大肠腺癌、３８例大肠腺瘤和２０例正常大肠粘膜组织的ＨＰＶ１６型ＤＮＡ序列。结果显示：三组病人ＨＰＶ１６型ＤＮＡ阳性率依次为４２％、３１．６％和０。大肠腺癌和腺瘤分别与正常大肠粘膜组织比较，ＨＰＶ１６型ＤＮＡ阳性率有显著差异（Ｐ＜０．０５）；大肠腺癌和腺瘤之间比较ＨＰＶ１６型ＤＮＡ阳性率无显著性差异（Ｐ＞０．０５）。但ＨＰＶ１６型ＤＮＡ阳性的１２例大肠腺癌组织均伴组织细胞异型增生。结果表明：ＨＰＶ１６型与国人的大肠腺癌有关。
To assess if human papilloma viruses (HPVs) are associated with human colorectal adenocarcinoma. Polymerase chain reaction (PCR) was used for the detection of HPV type 16 DNA in paraffinembeded tissue section from patients with colorectal adenocarcinoma (50 cases), colorectal adenoma (38 cases) and normal colorectal mucosa tissue (20 cases). Amplified products were analysed by agarose gel electrophoresis and Southern blot. HPV type 16 DNA sequences were found in 42%(21/50) of colorectal adenocarcinoma and 31.6%(12/38) of colorectal adenoma, but nil in normal colorectal mucosa. No statistical significant difference was evident between the contents of HPV 16 DNA in adenoma and carcinoma. Atypical hyperplasia was present in every patient with colorectal adenoma. In addition, HPV type 16 DNA was present in lymph node metastases of four patients as well. HPVs is implicated in pathogenesis of colorectal columnar cell tumors.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1998年第7期505-507,共3页
Chinese Journal of Clinical Oncology
关键词
大肠肿瘤
HPV16型
DNA
人乳头瘤病毒
PCR
Human papilloma virus (HPVs) Colorectal carcinomaPolymerase chain reaction (PCR) Southern blot
