摘要
Ｆｕｒａ－２显微荧光测钙技术研究发现，过氧亚硝基阴离子（ＯＮＯＯ－）作用于ＭＮ９Ｄ细胞，数ｓ内即可导致其胞内游离钙离子浓度（［Ｃａ２＋］ｉ）的急剧升高．胞外液换为无钙液或向胞外液中加入硝苯吡啶（Ｎｉｆｅｄｉｐ－ｉｎｅ）、二硫苏糖醇（ＤＴＴ）均可抑制ＯＮＯＯ－对［Ｃａ２＋］ｉ的影响，提示Ｌ－型钙通道的激活是ＯＮＯＯ－引起［Ｃａ２＋］ｉ升高的主要原因，ＯＮＯＯ－的这种作用可能与其氧化特性有关．Ｅｂｓｅｌｅｎ（２－苯基－１，２－苯并异硒唑－３（２Ｈ）酮）明显抑制ＯＮＯＯ－对［Ｃａ２＋］ｉ的影响，并且存在一定的剂量效应关系．
The mechanism of peroxynitrite (ONOO -) induced [Ca 2+ ] i increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by Fura 2 microfluorometric technique. Results showed that ONOO - caused a rapid increase of [Ca 2+ ] i when it was puffed to the cell. Removing Ca 2+ from the bath or using calcium channel antagonist (Nifedipine) greatly inhibited the [Ca 2+ ] i increase induced by ONOO -, which suggests that the opening of L Ca 2+ channel makes a great contribution to the [Ca 2+ ] i increase. The effect of sulfhydryl reductive agent (DTT) on ONOO - induced [Ca 2+ ] i increase suggests that ONOO - activating L Ca 2+ channel is partly related to its oxidative speciality. Ebselen shows an inhibiting effect on ONOO - induced [Ca 2+ ] i increase.
出处
《华中理工大学学报》
CSCD
北大核心
1998年第5期20-23,共4页
Journal of Huazhong University of Science and Technology
基金
国家自然科学基金
