摘要
采用 Fura- 2 / AM显微荧光测钙技术 ,检测培养新生大鼠单个心肌细胞内游离钙的浓度 ,并观察 1- (2 ,6 -二甲基苯氧基 ) - 2 - (3,4-二甲氧基苯乙氨基 )丙烷盐酸盐 (DDPH)对不同刺激〔去氧肾上腺素 ,高 K+ ,血管紧张素 (Ang II)〕所致心肌细胞钙超载的影响。结果表明 :1在含钙、无钙标准台氏液 ,DDPH 10 - 4 m ol· L- 1 对去氧肾上腺素(Phe) 10 - 4 mol· L- 1 所致钙增高有显著抑制作用。 2 DDPH 10 - 4 m ol· L- 1 对高 K+ 5 0 m mol· L- 1 引起的 [Ca2 + ]i增高有一定抑制作用 ,对 Ang 10 - 4 mol· L- 1 引起的 [Ca2 + ]i增高有部分拮抗作用。提示 :DDPH可拮抗 α1 受体调控的 Ca2 +通道 ,对钙库钙释放也有抑制作用 。
The effect of 1 (2,6 dimethylphenoxy) 2 (3,4 dimethoxyphenylethylamino) propane hydrochloride (DDPH) on the changes of intracellular free Ca 2+ concentration ([Ca 2+ ] i) induced by phenylephrine, KCl and Ang II was studied. The [Ca 2+ ] i in the cultured single ventricular cardiomyocyte from newborn rats was determined by using Fura 2/AM microfluorometric technique. The results showed that DDPH(10 -4 mol/L) could significantly inhibit 10 -4 mol/L phenylephrine induced elevation of [Ca 2+ ] i. Pretreatment of DDPH 10 -4 mol/L also reduced both KCl(50 mmol/L) and AngII (10 -4 mol/L) induced elevation of [Ca 2+ ] i. The results suggest that DDPH can decrease the elevation of [Ca 2+ ] i induced by phenylephrine through antagonizing receptor mediated Ca 2+ channel and regulating voltage dependent Ca 2+ channel respectively.
出处
《同济医科大学学报》
CSCD
2000年第3期199-201,共3页
Acta Universitatis Medicinae Tongji
基金
国家医药管理局新药基金资助项目!(No.CND-87040)
关键词
丙烷盐酸盐
心肌细胞
细胞培养
1-(2
6-dimethylphenoxy)-2-(3
4-dimethoxyphenylethylamino) propane
hydrochloride
calcium
free
cytosolic
myocardium
cells
cultured
