胎膜中MMP-2/TIMP-2表达与胎膜早破关系的研究被引量：1

Relationship between the expression of MMP-2/TIMP-2 in fetal membranes and premature rupture of membranes

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摘要目的探讨基质金属蛋白酶-2(MMP-2)及其抑制因子-2(TIMP-2)在自发性胎膜早破孕妇的胎膜中的变化及其意义。方法收集胎膜早破确诊病例30例,同期收集正常妊娠择期剖宫产30例。采用免疫组织化学PV9000法检测MMP-2及TIMP-2在胎膜中的表达。结果1.所选两组标本感染率比较差别无统计学意义(χ2=2.857,P=0.091);2.PROM组胎膜组织结构明显疏松,羊膜与绒毛膜之间的区域普遍较对照组增宽;3.早破组与正常组在HE染色后着色深浅有异,早破组略显淡;4.MMP-2在胎膜早破产妇胎膜中的表达高于正常妊娠剖宫产组阳性率分别为100%,46.67%差异有显著性(U=5.849,P=0.000);5.TIMP-2在胎膜早破产妇胎膜中的表达低于正常妊娠剖宫产组,差异无显著性(U=1.690,P=0.091)。结论胎膜早破的发生可能与MMP-2的表达上调有关。 Objective： To explore the changes and significance of matrix metalloproteinase2 and tissue inhibitors of matrix metaUo- proteinase2 in fetal membranes of pregnant women with premature rupture of membranes （PROM）. Methods ： Choose groups 1 with premature rupture of membrane （ n = 30） , group 2 undergoing elective cesarean section （ n = 30） . The expression of MMP - 2, TIMP- 2 in fetal membranes was detected by immunohistochemistry PV9000 method. Results： 1. The difference between two group＇ s infection rate hasn＇t statistically significant. 2. The structures of PROM group are obviously puff. 3. The areas between amniotic membrane and chorionie veside in PROM group are generally wider than that in control group. 4. The positive expression in the fetal membranes of PROM groups were higher than that in normal pregnant women group. It was 100% and 46. 67% , respectively, there were significant differences （ U =5. 849, P = 0. 000 ）. 5. The expression of TIMP - 2 in the fetal membranes of PROM groups were lower than that in normal pregnant women group. But there was no significant difference （ U = 1. 690, P = 0. 091 ）. Conclusion The increase expression of MMP - 2 maybe related to the occurrence of PROM.

作者张莉杨海澜冀静李秀芳武馥珍

机构地区山西医科大学第一附属医院产科

出处《中国优生与遗传杂志》 2009年第4期58-59,62,共3页 Chinese Journal of Birth Health & Heredity

关键词基质金属蛋白酶-2 基质金属蛋白酶抑制因子-2 胎膜早破免疫组织化学 Matrix metalloproteinase - 2 Tissue inhibitor of matrix metalloproteinase - 2 Premature rupture of fetal merebranes Immunohistochemistry

分类号 R714.433 [医药卫生—妇产科学]

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参考文献11

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