摘要
目的探讨检测孕妇血清中基质金属蛋白酶-9(MMP-9)和血清及羊水中肿瘤坏死因子-α(TNF-α)在诊断胎膜早破孕妇绒毛膜羊膜炎中的作用。方法选择2004-06-2004-12在汕头市潮南区人民医院和汕头大学医学院第一附属医院产科住院分娩的胎膜早破患者42例为研究组,并选择同期正常40例孕妇为对照组,采用酶联免疫吸附法和高敏感的放射免疫法检测血清的MMP-9和血清及羊水中的TNF-α水平,同时行产后胎膜病理检查。结果胎膜早破孕妇羊水中TNF-α和血清中MMP-9水平明显高于对照组,差异显著(P<0·05);但血清中的TNF-α与对照组比较差异无显著性(P>0·05)。血清中MMP-9水平在重度和轻中度绒毛膜羊膜炎组差异显著(P<0·05);而羊水中TNF-α差异无显著性(P>0·05)。结论测定母体血清的MMP-9含量和羊水中TNF-α水平,可作为胎膜早破患者是否患有绒毛羊膜炎的辅助诊断指标,尤其是血清的MMP-9含量的高低,还可以判定绒毛膜羊膜炎的严重程度。
Objective To explore the effect of matrix metalloproteinase-9 in serum and tumor necrosis factor-α in serum and amniotic fluid in predicting chorioamnionitis in patients with premature rupture of membranes （PROM）. Method The levels of MMP-9 in serum and TNF-α in serum and amniotic fluid were measured by radioimmunoassay and enzyme-labeled immunosorbent assay（ELISA） in 42 cases with premature rupture of membranes as study group and 40 casess normal fulltermed pregnant women as control group,who were selected from People＇s Hospital of Chaonan District of Shantou and the First Hospital of Medical College of Shantou University From June 2004 to Dec 2004. Result The levels of TNF-α in amniotic fluid and MMP-9 in serum in study group were significantly higher than those in control group （P 〈0. 05）, but the levels of TNF-α in serum were not （P 〉0. 05）. The levels of MMP-9 in serum in serious chorioamnionitis were significantly higher than that in diminutive chorioamnionitis （P 〈 0. 05 ）, but the levels of TNF-α in amniotic fluid were not （ P 〉 0.05）. Conclusion The levels of TNF-α in amniotic fluid and MMP-9 in serum are valuable clinical indices for identification of chorioamnionitis in patients with PROM. The levels of MMP-9 in serum can also assess the degree of chorioamnionitis.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2006年第6期433-434,共2页
Chinese Journal of Practical Gynecology and Obstetrics
