摘要
以明胶、果胶、海藻酸钠、氯化钙和壳聚糖为壁材,采用乳化法双层包埋制备双歧杆菌微胶囊。制备的微胶囊粒径在10～30μm。检测结果表明,微胶囊内活菌数可达到109CFU/g以上,菌体包埋率可达到82.24%。经模拟胃酸、胆汁酸处理后活菌数仍在108CFU/g以上,对酸有很高的耐受力;经人工肠液处理15min,微胶囊几乎全部崩解,肠溶释放率可达到95.81%。通过经典的加速实验证明微胶囊的活菌贮藏稳定性较好,室温下贮藏1年其活菌数仍可以保持在108CFU/g以上。
With gelatin, pectin, alginate sodium, calcium chloride and chitosan as wall materials, Bifidobacterium bifidum microcapsules were prepared by emulsification and double-layer embedment method. The determination results showed the diameter of microcapsule particles is 10 to 30μm, the viable cell count is more than 109 CFU/g, and the embedding rate reaches 82.24%. After stimulated gastric solution or bile acid treatment, the viable cell count of Bifidobacterium bifidum in microcapsule is more than 10^9 CFU/g, which indicates its high acid tolerance. After the treatment of stimulated intestinal solution for 15 min, almost all microcapsules disintegrate and the release rate reaches 95.81%. The classic accelerating test proved that the Bifidobacterium bifidum microcapsules have good stability for storage. After one-year storage at room temperature, the viable cell count is still over 10^8 CFU/g.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第7期141-144,共4页
Food Science
基金
哈尔滨市攻关项目(0114211066)
大庆市产业化资助项目
关键词
双歧杆菌
乳化法
微胶囊
活菌数
Bifidobacterium bifidum
emulsification method
microcapsule： viable cell count
