摘要
以海藻酸钠和壳聚糖两种载体对木聚糖酶进行固定化,研究游离酶和固定化酶的性质。结果表明,游离酶的Km=0.546mg/L,以海藻酸钠和壳聚糖为载体的固定化酶的Km分别为38mg/L和0.342mg/L。尽管以壳聚糖固定的酶的最适pH与游离酶相同(都为5.0),但前者的适宜pH范围明显变宽;而经海藻酸钠固定的酶,最适pH向酸性范围移动0.5个pH;两种载体固定的酶最适温度与游离酶相比,都从40℃提高到50℃。实验还表明,固定化酶提高了游离酶的贮藏稳定性。
Xylanase was immobilized by chitosan and sodium alginate as carrier respectively and the properties of free xylanase and immobilized xylanase were investigated. The results indicated that the Km value of free xylanase was 0.546 mg/L, and the Km values of immobilized xylanase with sodium alginate as carrier and chitosan as carrier were 38 mg/L and 0.342 mg/L respectively. The best pH value for immobilized xylanase with chitosan as carrier and free xylaaase was the same （both as 5.0）, but the former one had wider pH range. The optimal pH value for immobilized xylanase with sodium alginate as carrier shifted 0.5 pH value to acidity direction. The optimal temperature of immobilized xylanase increased from 40 ℃ to 50 ℃ compared with free xylanase. The experiments also showed that the immobilized xylanase had better storage stability than free xylanase.
出处
《酿酒科技》
北大核心
2007年第2期35-37,共3页
Liquor-Making Science & Technology
基金
湖北省教育厅项目资助(D2005118004)
武汉工业学院大学生科研立项重点项目资助。
关键词
木聚糖酶
壳聚糖
海藻酸钠
固定化
xylanase
chitosan
sodium alginate
immobilization
