摘要
用放射免疫测定和免疫组化法观察缺氧对培养的小牛肺动脉内皮细胞（ＰＡＥＣ）和肺动脉平滑肌细胞（ＰＡＳＭ）自分泌心钠素（ＡＮＰ）、血管紧张素Ⅱ（ＡＴⅡ）和内源性洋地黄因子（ＥＤＬＦ）的影响。无氧培养２４ｈ末，ＰＡＥＣ分泌ＡＮＰ减少４３．５％（Ｐ＜０．００１），ＡＴⅡ和ＥＤＬＦ呈明显负相关（ｒ为－０．８８和－０．７８６，Ｐ＜０．０１），细胞内ＡＮＰ阳性颗粒也显著减少（Ｐ＜０．００１）；ＰＡＳＭ分泌ＡＮＰ无显著改变，而细胞内ＡＮＰ明显减少（Ｐ＜０．００１），ＰＡＳＭ分泌ＡＮＰ与ＥＤＬＦ呈正相关（ｒ＝０．７２２，Ｐ＜０．０１），与ＡＴⅡ呈负相关（ｒ＝－０．８６５，Ｐ＜０．０１）。结果表明：缺氧时ＰＡＥＣ和ＰＡＳＭ的ＡＮＰ、ＡＴⅡ和ＥＤＬＦ等自分泌的改变可能参与缺氧性肺血管收缩及结构的改建。
The effects of hypoxia on secretion of atrial natriuretic peptide (ANP), angiotensin Ⅱ (ATⅡ) and endogenous digitalislike factor (EDLF) by pulmonary artery endothelial cells (PAEC) and pulmonary artery smooth muscle cells (PASM) were investigated by using immunohistochemistry and RIA method. In response to anoxia for 24 h, the release of ANP from PAEC decreased by 43.5% (P<0.001), whereas the secretion of ATⅡ and EDLF by PAEC increased significantly (P<0.001). The release of ANP from PAEC was negatively correlatied with ATⅡ and EDLF (r=-0.88 and -0.786 respectively, P<0.01). Hypoxia unaffected the secretion of ANP by PASM, but the release of ATⅡ decreased (P<0.001) and EDLF increased significantly by PASM under anoxia (P<0.01) . The ANP release from PASM was positively correlated with EDLF (r=0.722, P<0.01) and negatively correlated with ATⅡ(r=-0.865, P<0.01). It was indicated that autocrine and paracrine of ANP, ATⅡ and EDLF might play important roles in the development of hypoxic vasoconstriction and pulmonary vascular remodeling.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1998年第1期32-34,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
关键词
缺氧
肺动脉
内皮
血管紧张素
cell hypoxia
endothelium
pulmonary artery
angiotensin
