摘要
肺血管局部的肾素血管紧张素系统在缺氧性肺动脉高压的发生中所起的作用尚不清楚。为此，动态观察了缺氧对培养的新生小牛肺动脉内皮细胞（ＰＡＥＣ）的血管紧张素转换酶（ＡＣＥ）活性的影响。结果发现：培养的ＰＡＥＣ在２．５％Ｏ２缺氧１．５ｈ末，培养液ＡＣＥ活性明显增高（与常氧组及０％Ｏ２组相比Ｐ＜０．００１）；随缺氧时间延长，３ｈ～１２ｈ，ＡＣＥ活性与１．５ｈ相比有所降低，但仍高于常氧组（Ｐ＜０．０５）；２４和４８ｈ，ＡＣＥ活性又大幅度升高（与常氧组及０％Ｏ２相比Ｐ＜０．００１）。０％Ｏ２缺氧条件下，ＡＣＥ活性仅在６ｈ和１２ｈ高于常氧组，而在２４ｈ低于常氧组（Ｐ＜０．０５），其它时点与常氧组相比差异不明显。０％Ｏ２条件下培养２４ｈ后，ＰＡＥＣ细胞内的ｃＧＭＰ含量明显降低（与常氧组相比Ｐ＜０．０５）。上述结果表明缺氧可引起ＰＡＥＣ的ＡＣＥ活性改变。
The effects of hypoxia on the angiotensin converting enzyme (ACE) secretion were dynamically observed in cultured pulmonary arterial endothelial cells (PAECs) of new born bovine to explore the role of local renin angiotensin system in local pulmonary artery playing in the occurrence of hypoxic pulmonary arterial hypertension. It was found that: The ACE activity was significantly decreased when PAECs were incubated under 2.5% O 2 hypoxic condition for 1.5 h ( P <0.001 vs control and 0% O 2 groups) and further decreased from 3 to 12 h but still remained high ( P <0.05 vs the control) but significantly increased from 24 to 48 h ( P <0.001 vs control and 0% O 2 groups). Under 0% O 2 hypoxic condition, it was only significantly higher at the 6th and 12th hour and lower at the 24th hour after incubation than that in the control group ( P <0.05) and the cGMP content in PAECs was significantly 24 hours after the incubation (P<0.05vsthe control). Our findings suggest that hypoxia can cause the changes of ACE activity in PAECs, which might participate in the occurrence of hypoxic pulmonary arterial hypertension
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1996年第6期512-514,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
缺氧
内皮细胞
肺血管
血管紧张素
转换酶
hypoxia
endothelial cell
pulmonary artery
angiotensin converting enzyme
