摘要
对２５例大肠癌根治术患者的新鲜标本进行细胞分离和原代培养，采用噻唑氮蓝（ＭＴＴ）比色分析法和氚胸腺嘧啶核苷（３ＨＴｄＲ）掺入法，在体外观察５肽胃泌素（ＰＧ）对原代培养大肠癌活细胞数（吸光度，Α值）和ＤＮＡ合成（脉冲数，ＣＰＭ值）的影响。结果：ＰＧ组高、中及低分化腺癌活细胞数和ＤＮＡ合成均明显高于对照组（Ｐ＜０．０１，Ｐ＜０．０５）。出现明显促细胞生长所需ＰＧ浓度高分化腺癌组为０．３９０６μｇ／ｍｌ，而中、低分化腺癌组均为６．２５００μｇ／ｍｌ。本研究结果提示：胃泌素可促进人大肠癌细胞的增殖，尤以高分化腺癌对胃泌素更敏感。这为大肠癌患者特别是高分化腺癌应用胃泌素受体拮抗剂进行内分泌治疗提供了实验依据。
The effects of pentagastrin (PG) on the viable cell count (Α value) and the synthesis of DNA (CPM value) of primary cultured large bowel carcinoma cells in 25 patients were evaluated in vitro by MTT assay, 3H TdR incorporation. The results showed that Α value and CPM value in well, moderately and poorly differentiated carcinoma cells were higher than normal control ( P <0.01, P <0.05). The proliferative effect was significant at a dose of 0.3907 μg/ml in well differentiated carcinoma cells, and at a dose of 6.2500μg/ml in moderately and poorly differentiated carcinoma cells. These indicat that PG has the proliferative effect on large bowel carcinoma cells. These results provide an experimental foundation for the endocrine therapy for patients with large intestine carcinoma, especially by using gastrin receptor antagonists for well differentiated carcinoma.
出处
《中国普外基础与临床杂志》
CAS
1998年第3期143-145,共3页
Chinese Journal of Bases and Clinics In General Surgery
