摘要
用ＭＴＴ比色分析法和流式细胞术在体外研究了５肽胃泌素（ｐｅｎｔａｇａｓｔｒｉｎ，ＰＧ）及胃泌素受体拮抗剂丙谷胺（ｐｒｏｇｌｕｍｉｄｅ，ＰＧＬ）对人大肠癌细胞株ＳＷ４８０的作用。结果显示：ＰＧ能促进ＳＷ４８０细胞生长；ＰＧＬ单用时对ＳＷ４８０细胞没有影响，当ＰＧ与ＰＧＬ合用时，ＰＧＬ呈剂量依赖地拮抗ＰＧ的作用，ＰＧＬ在３２μｇ／ｍｌ以上时，拮抗作用趋于恒定。提示胃泌素的作用是通过其受体发挥的。ＰＧ促进ＳＷ４８０细胞合成ＤＮＡ、蛋白质，促进ＳＷ４８０细胞从Ｇ０／Ｇ１期过度到Ｓ期和Ｇ２Ｍ期；ＰＧＬ完全拮抗这种作用。
Human SW480 colonic cancer cell line was evaluated for its growth response to pentagastrin, gastrin receptor antagonist proglumide (PGL) in vitro by MTT assay and flow cytometry. The results showed that gastrin possessed a proliferative effect on SW480 cell, PGL alone had no obvious effect on SW480 cell, but it inhibited gastrin induced growth of SW480 cell with dosage dependent when it was used with gastrin, its inhibitive effect did not steadly increase at a dose>32 μg/ml. This suggests that effect of gastrin is achieved via gastrin receptor. Gastrin promoted the sythesis of DNA, protein and triggered the cancer cell shifting from phase G 0/G 1 to phase S, G 2M. PLG inhibited the effect of gastrin, it suggests that gastrin possessed a proliferation on SW480 cell at post receptor is achieved by the effect of gastrin on cell cycle.
出处
《中国普外基础与临床杂志》
CAS
1998年第1期1-3,共3页
Chinese Journal of Bases and Clinics In General Surgery
关键词
胃泌素
受体拮抗剂
大肠癌
Gastrin Gastrin receptor antagonist In vitro Large intestinal carcinoma Flow cytometry
