摘要
目的 探讨丙谷胺在胃泌素促进人结肠癌细胞株SW480中的作用及其机制。方法 采用噻唑氮蓝 (MTT)比色分析法 ,3H 肌醇掺入法 ,Ca2 +荧光测定技术和γ 32 P ATP掺入法 ,在体外观察丙谷胺 (PGL)对 5肽胃泌素 (PG)促人结肠癌细胞株SW480增殖的影响。结果 PG +PGL组的SW480活细胞数 (viablecellcount,VCC)降低 ,与对照组相比 (P <0 0 1) ,与PG组相比 (P <0 0 1) ;PG +PGL组细胞内三磷酸肌醇 (inositol 1,4,5 triphosphate,IP3)、Ca2 +浓度降低 ,与PG组相比 (P <0 0 1) ,与对照组相比 (P >0 0 5 ) ;PG +PGL组膜蛋白激酶C(proteinkinaseC ,PKC)活性降低 ,与PG组相比 (P <0 0 5 ) ,与对照组相比 (P >0 0 5 )。结论 本研究提示丙谷胺可能通过肌醇质脂信号通路抑制胃泌素促进人结肠癌细胞株SW480的增殖 。
Objective To investigate the role and clinical significance of proglumide (PGL) in gastrin stimulating cell proliferation on human colon cell line SW480.Methods Effects of proglumide on the viable cell count ( A value), IP 3 (CPM value), [Ca 2+ ] i and PKC activity of human colon cell line SW480 were evaluated in vitro by MTT assay, 3H myo inositol incorporation, fluorescence measurements and γ 32 P ATP incorporation. Results The results showed that A value of PGL+PG group is lower than that of PG or control group ( P <0 01 in both groups); The concentration of IP 3 or [Ca 2+ ] i in PGL+PG group is lower than that in PG group ( P <0 05). Conclusion These results indicate effects of proglumide may be through the phosphoinositide signalling pathway on gastrin stimulating cell proliferation in human colon cell line SW480. It will provide an experimental evidence for antisingnal transduction therapy for patients with colon cancer.
出处
《肿瘤》
CAS
CSCD
北大核心
2001年第2期88-90,共3页
Tumor
