摘要
目的：研究麝香配伍冰片对局灶性脑缺血再灌注损伤大鼠的脑保护作用。方法：用颈内动脉线栓法建立大鼠局灶性脑缺血再灌注模型，检测脑梗死体积，并比较其神经功能行为积分；于湿重法观察脑含水量的变化，通过收集透出脑血管外的伊文思蓝（EB）来示踪血脑屏障（BBB）的变化。结果：脑缺血2h再灌注24h，与假手术组比较模型组脑梗死体积显著增大（P〈0．01），神经功能行为积分显著升高（P〈0．01）；麝香冰片组脑梗死体积较模型组缩小（P〈0．05），同时，麝香冰片组、麝香组可使大鼠神经功能行为积分下降（P〈0．05～0．01），其中以麝香冰片组作用最明显；模型组较假手术组脑含水量及EB含量明显增加（P〈0．01），血脑屏障遭到破坏；麝香冰片组脑含水量较模型组明显降低（P〈0．05），麝香冰片组及冰片组EB含量较模型组明显降低（P〈0．01）。结论：麝香配伍冰片可减少局灶性脑缺血再灌注大鼠脑梗死体积、改善脑缺血后神经行为症状，有效降低脑缺血再灌注后脑含水量及血脑屏障的通透性，对局灶性脑缺血再灌注损伤大鼠有一定的脑保护作用。
Aim：To investigate the effect of Musk with Borneol on brain damage in the rat model of cerebral focal ischemia with reperfusion. Methods：The model of MCAO-reperfusion was induced by the method of nylon monofilament via the internal carotid artery. Technique of image analysis was used to calculate the volume of cerebral infarction and evaluate the scores of nerves function; Method：of dry and wet weight was used to observe water content and Evans blue （EB） was used to observe the changes of BBB permeability. Results：After cerebral ischemia for 2 hours and reperfusion for 24 hours, the volume of cerebral infarction of the model group increased significantly （ P 〈 0.01 ）, the scores of the function of nerves increased （ P 〈 0.01 ） ; the volume of cerebral infarction of the Musk with Borneol group was significantly lower than that of the model group （ P 〈 0.05） ; the scores of the function of nerves of Musk with Borneol group, Musk group were significantly lower than that of the model group （ P 〈 0.05 - 0. 01 ） ; the content of water and EB in the model group increased significantly compared with sham operation group（ P 〈 0.01 ）, which indicated the BBB was damaged ; The water content in Musk with Borneol group was significantly lower than that of the model group （ P 〈 0.05） and the content of BBB in Musk with Borneol group and Borneol group was significantly lower than that of the model group（P 〈 0.01）. Conclusion： Musk with Bomeol can decrease the volume of cerebral infarction and improve Nervous symptom, decrease the water content and permeability of BBB after focal cerebral ischemia with reperfusion, therefore protect the brain after focal cerebral ischemia with reperfusion.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第2期42-45,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
麝香
冰片
脑缺血再灌注
脑保护
血脑屏障
Musk
Borneol
cerebral ischemia-reperfusion
brain protection
BBB
