摘要
采用聚丙烯酰胺梯度凝胶电泳的方法,对人工选育池蝶蚌的3个世代的8种组织(心、肝、肾、外套膜、性腺、鳃、斧足、闭壳肌)的酯酶(EST)和苹果酸脱氢酶(MDH)进行研究,比较了不同组织的相对迁移率及酶活性大小。结果表明,池蝶蚌的EST和MDH同工酶具有明显的组织差异性,不同世代间没有明显的差异性,表明其经人工选育后已初步成为一个纯系,遗传性状趋于稳定。
In the study, two isozymes （ EST and MDH） in 8 tissues （ heart, liver, kidney, mantle, sex gland, gill, todium and adductor muscle ） of three generations of Hyriopsis schlegelii were studied by vertical gradient gel elec trophoresis on polyacrylamide. The loci expression and phenotypes of the two isozymes were analysed, and the activity and relative mobility （Rf） of these two isozymes in different tissues were compared. The results show that the EST and MDH electrophoresis patterns exhibited, an apparent tissue - specificity while there were no significant differences among the different generations. It indicates that the Hyriopsis schlegelii has become a pure strain after artificial selected breeding, and its genetic characteristics tend to be stable.
出处
《水生态学杂志》
北大核心
2008年第6期48-52,共5页
Journal of Hydroecology
关键词
池蝶蚌
同工酶
人工选育
Hyriopsis schlegelii
isozyme （ EST and MDH）
selected breeding
