摘要
用聚丙烯酰胺梯度凝胶电泳分析了鲢×鳙、鲢×团头鲂的二倍体和异源三倍体杂种,以及同源三倍体鲢及其亲本种的8种蛋白质和同工酶的表型。二倍体杂交子代的基因表达是多样的,这些多样的表达可能与双亲的这些蛋白质和同工酶的表型差异有关。如与二倍体杂交子代相比,鲢×鳙异源三倍体的肌肉蛋白和血清蛋白有较一致的表型。此外在两种异源三倍体中EST和MDH同工酶的表型接近母本。这些结果表明异源三倍体由于增加了一套母本染色体组,基因的表达较接近母本或趋向一致。同源三倍体鲢与二倍体鲢的肌肉蛋白、血清蛋白、G3PDH、MDH和LDH同工酶表型没有区别。
The protein and isozyme phenotypes of diploid and allotriploid hybrids of female silver carp×male bighead carp and female silver carp×male blunt snout bream, autotriploid silver carp and their parental species were analysed by gradient gel electrophoresis on polyacrylamide. The gene expression of diploid F_1 hybrids was variable, and the variable expressions might be related to the phenotypic difference in proteins and isozymes in both parental species. In comparison with diploid F_1 hybrids, the phenotypes of muscle proteins and serum proteins were basically identical in the allotriploids of silver carp and bighead carp. In addition, the phenotypes of EST and MDH in both allotriploids were close to those of maternal silver carp. These results suggested that the gene expression of allotriploid should be close to the maternal species and have a stable and identical tendency due to the increase of one maternal genome. The phenotypes of muscle proteins, serum proteins, G3PDH, MDH and LDH isozymes were not different between autotriploid and diploid silver carp.
出处
《水生生物学报》
CAS
CSCD
北大核心
1993年第4期293-297,共5页
Acta Hydrobiologica Sinica
关键词
鲢
远缘杂交
三倍体
同功酶
鱼纲
Silver carp, Distant hybridization, Allotriploid, Autotriploid, Isozyme and protein
