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中国HIV感染者细胞毒性淋巴细胞与CD4^+CD25^+调节性T细胞相关性研究 被引量：1

Correlation between CD4^+CD25^+Foxp3^+ regulatory T cells and cytotoxic lymphocytes in HIV infected patients
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摘要 目的:对中国HIV感染者NK细胞、CD8+T细胞及胞内穿孔素、颗粒酶-B表达与CD4+CD25+Foxp3+调节性T细胞水平的相关性进行研究,探讨调节性T细胞在HIV感染中的作用机制。方法:选取73名HIV/AIDS患者(长期不进展组、无症状HIV组、AIDS组),应用流式细胞仪胞内染色技术检测NK细胞、CD8+T细胞数量及胞内穿孔素、颗粒酶-B表达水平,分析其与CD4+CD25+Foxp3+调节性T细胞水平的相关性。结果:CD4+CD25+Foxp3+调节性T细胞百分率与NK细胞、CD8+T淋巴细胞数量呈明显负相关(P<0.01),与CD8+T细胞内穿孔素、颗粒酶-B表达百分率呈明显正相关(P<0.05),与NK细胞内穿孔素、颗粒酶-B表达水平绝对值负相关(P<0.01),与CD8+T细胞内颗粒酶-B表达绝对值呈明显负相关(P<0.01),与CD8+T细胞内穿孔素表达绝对值无明显相关性(P>0.05)。CD4+CD25+Foxp3+调节性T细胞绝对值与CD8+T细胞内穿孔素、颗粒酶-B表达百分率呈明显负相关(P<0.05)。结论:中国HIV感染者细胞毒性淋巴细胞数量功能的变化与调节性T细胞显著相关,提示高水平的调节性T细胞可能与细胞毒性淋巴细胞耗竭相关,可能为导致疾病进展的原因之一。 Objective：To detect the relationship between the frequencies of CD4^＋CD25^＋Foxp3^＋ regulatory T cells and cytotoxic lymphocytes in HIV infected patients in China,in order to study the mechanism of regulatory T cells in HIV infection.Methods：73 untreated HIV-1 infected patients and 16 normal controls were enrolled and the frequencies of CD4^＋CD25^＋Foxp3^＋ regulatory T cells,NK cells,CD8^＋T cells and granzyme-B,perforin expression of cytotoxic lymphocytes were detected by flow-cytometry.Results：The frequency of CD4^＋CD25^＋Foxp3^＋Treg cells was inversely related to those of NK,CD8 cells（NK cell：r=-0.502,P〈0.01;CD8^＋T cell：r=-0.437,P〈0.001）.It was positive correlated with the proportion of granzyme-B,perfor in expression of CD8^＋ T cells but inversely correlated with absolute count of granzyme-B,perforin expression of NK cells and perforin expression of CD8 cells.The absolute count of CD4^＋CD25^＋Foxp3^＋Treg cells was inversely related to the proportion of granzyme-B,perforin expression of CD8 cells.Conclusion：The frequency of CD4^＋CD25^＋Foxp3^＋ regulatory T cells are correlated with the change of the number and perforin,grazyme-B expression of cytotoxic lymphocytes,which indicates the high level of regulatory T cells may attribute to the exhausion of cytotoxic lymphocyes,thus affect the disease progression of HIV infection.
作者 张子宁 姜拥军 刘静 金鑫 孙国权 施万英 张旻 王亚男 韩晓旭 尚红
机构地区 中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2008年第12期1125-1129,共5页 Chinese Journal of Immunology
基金 国家自然科学基金(30600532) 国家重点基础研究发展计划973计划(2006CB504206)资助
关键词 HIV-1 调节性T细胞 细胞毒性淋巴细胞 颗粒酶 穿孔素 HIV-1 Regulatory T cell Cytotoxic lymphocytes Granzyme Perforin
分类号 R512.91 [医药卫生—内科学]
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参考文献20

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二级参考文献15

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中国免疫学杂志
2008年 第12期

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