摘要
以珍珠梅的腋芽茎段为外植体,探索不同杀菌时间、不同培养基、不同激素配比和浓度对珍珠梅离体培养的影响。结果表明,用0.1%HgCl2溶液,加入Tween-20,灭菌5 min处理,外植体染菌率较低,为4.8%;初代培养基以MS+6-BA0.6 mg/L的萌芽率最好,达100%,增殖倍数最高,为3.7;珍珠梅继代培养适宜的培养基为MS+6-BA0.6 mg/L+NAA0.2 mg/L,其萌芽率、增殖倍数分别为82.4%,3.7;生根培养基以1/4MS+IBA 0.2 mg/L+IAA0.2 mg/L较好,生根率、平均根数分别为85%,8,且多为实生根。
To explore effects of different time of sterilization,culture mediums,hormones combination and concentrations on vitro culture of Sorbaria sorbifoli A. Br by using stems of axillary buds as explants. The results showed that the medium added Tween-20 and explants adopted solution of 0.1% HgCl2 steriled for 5 min, the rate of germination was lower by 4. 8%. Explants of primary culture in medium MS＋6-BA 0.6 mg/L grew better. The rate of germination and proliferation times were 100% and 3.7 respectively. The better suitable for secondary culture of explants was culture medium of MS＋6-BA 0.6 mg/L＋NAA 0.2 mg/L. Rate of germination and proliferation times of Sorbaria sorbifoli A. Br were 82.4% and 3.7 respectively. Roots were effectivly induced with culture medium 1/4MS＋IBA 0.2 mg/L＋IAA 0.2 mg/L, rate of rooting and average number of roots were 85% ,and 8 respectively.
出处
《新疆农业大学学报》
CAS
2008年第6期38-41,共4页
Journal of Xinjiang Agricultural University
基金
新疆自然科学基金项目(200821105)
关键词
珍珠梅
茎段
离体培养
Sorbaria sorbifoli A. Br
stem segment
vitro culture
