摘要
目的：探讨高效毛细管电泳法（HPCE）在测定桑叶中芦丁含量的作用。方法：选择新疆不同采集期桑叶。HPCE条件：熔融石英毛细管50cm×50μm，uncoated；硼砂和磷酸二氢钠缓冲溶液20mmol／L，其中含15％甲醇溶液，pH8．5；分离电压20kV（＋）-（-）；电泳时间12min；电泳温度25℃；压力进样0．5psi×10s；柱上检测UV254nm（二极管阵列检测器）。结果：样品可在10min内出峰，测得蒜氨酸浓度在O～80μg／ml范围内与峰面积的线性关系良好（r=0．9945），平均回收率为98．9％，批内RSD为3．9％。结论：高效毛细管电泳法准确、简单、快捷、重现性好。
Objective： To establish a HPCE method to determine the content of rutin in mulberry leaves. Methods： HPCE mettlod： bare fused-silica capillary 50 cm× 50 μm; borax buffer 20 mmol/L containing 15% methanol at pH8.5; running voltage 20 KV; polarity from anode （inlet） to cathode （outlet）; separate time 12 mini temperature 25℃ ; sampling 0.5 psi×10 s; on line detection at 254 nm （PDA）. Results： The sample could be separated in 10 min. The linearity relationship of rutin concentration and peak value was good in the range of 0-80 μg/ml. The related coefficient was r =0. 9945, the average recovery of sample for alliin was 98.9%, and the RSD of inter-day was 3.9%. Conclusion： This methods is simple, quick, accurate and with satisfactory recoveries.
出处
《新疆医科大学学报》
CAS
2008年第10期1361-1362,共2页
Journal of Xinjiang Medical University
关键词
高效毛细管电泳
桑叶
芦丁
HPCE
mulberry leaves
rutin
content determination
