摘要
用氮蓝四唑（ＮＢＴ）光化还原法，对从桑叶、桑嫩芽、桑枝、桑皮、蚕沙中提取的黄酮类化合物清除超氧离子自由基Ｏ·２的效果进行了测定．结果表明，这类化合物有较强的清除作用，在浓度为５μｇ·ｍＬ－１时，从桑嫩叶、桑枝、桑皮中提取的这类化合物其清除率分别为６８．５％、８３．１％、５６．８％，其清除作用比芸香甙（芦丁）、抗坏血酸强．桑叶黄酮类化合物的清除率为４７．２％，比抗坏血酸强．蚕沙中提取的黄酮类化合物在浓度为６μｇ·ｍＬ－１时也有很强的清除作用．作者在文末讨论了桑树黄酮类化合物清除Ｏ·２的机理．
The scavenging superoxide free radical effects of mulberry leaves, mulberry tender shoot,mulberry branch,mulberry bark and silkworm dung were studied by nitro blue tetrazolium(NBT) photo reduction. The results showed that these flavonoids aqeious extracts had strong scavenging effect on superoxide free radical. The scavenging effect had relation to the content of the flavonoids. When the concentration of flavonoids extracted from mulberry tender shoot,mulberry branch and mulberry bark were 5 μg·mL -1 ,scavenging rate were 68.5%,83.1%,56.8% respecttively. Their scavenging effect are much stronger than rutin and ascorbic acid. When the concentration of flavonoid extractted from mulberry leaves was 5 μg·mL -1 its scavenging rate was 47.2%. Its scavenging effect was much stronger than ascorbic acid. When the concentreation of flavonoids extracted from silkworm dung was 6 μg·mL -1 ,it had strong scavenging effect. The artical also discussed the mechanism of the scavenging superoxide free rsdical effect with mulberry flavonids.
基金
浙江省教委资助项目
