摘要
目的:用MTT法检测临床常用的4种进口铸造合金(镍铬合金、钛合金、钴铬合金、金合金)的细胞毒性。方法:用MTT法测定人牙龈膜成纤维细胞在不同合金浸提液中培养2d、4d、7d的吸光度(A值),计算细胞相对增殖率(RGR),判断细胞毒性的级别。结果:各组细胞不同时间点相对增殖率均在96%～106%之间,镍铬合金的细胞毒性评级为1级,其余3种合金为O级。结论:4种铸造合金的细胞毒性均在临床应用的允许范围内。
Objective： To identify the cytotoxicity of these four dental casting alloys. Method： MIT assay method was used to evaluate the cytotoxicity of four alloys to human gingival fiberblast cells isolated from healthy periodontal ligaments of subjects at the 2nd d,4th d and 7th d. Result：The relative growth rates of the HPDLF are above 90% at the 2 d,4 d, and7 d： cytotoxicity grade is 0 and 1. Through the statistic analysis,there is no statistical difference between the 4 groups and the negative control group G~ 〉 0.05）.Conclusion： Cytotoxicity of four alloys is grade 0 or 1, which meets the safety standard.
出处
《临床口腔医学杂志》
2008年第11期649-651,共3页
Journal of Clinical Stomatology
基金
云南省教育厅项目基金(项目编号:5Y0367C)
