摘要
以AIBN为引发剂,AET·HCl为链转移剂,通过自由基聚合制备了具有温敏性的P(NIPAM-co-NMAM)-g-(PLLA-g-MAAH)接枝共聚物.通过NMR、FTIR和GPC分析研究了生成的接枝共聚物的化学结构、相对分子质量及相对分子质量分布;利用DLS和TEM手段对产物的粒子尺寸、分布及其形貌进行了研究;利用透光率及表面张力测定研究了其温敏行为及胶束化行为.实验结果表明,合成的P(NIPAM-co-NMAM)-g-(PLLA-g-MAAH)接枝共聚物在水溶液中形成近似球形胶束,平均粒子尺寸约20 nm,具有最低临界溶液温度约42℃,临界胶束浓度约28 mg/L.MTT试验表明合成的共聚物无细胞毒性或有轻微毒性,有望成为一种有潜力的生物医用材料.
Thermosensitive P（NIPAM-co-NMAM）-g-（PLLA-g-MAAH） graft copolymers were synthesized via a free radical polymerization route using 2, 2＇-azobisisobutyronitrile （AIBN） as an initiator and 2- aminoethanethiol hydrochloride （AET. HCl） as a chain transfer agent. The structure, molecular weight and polydispersity of the copolymer were characterized by NMR, FTIR and GPC. The morphologies, size and size distribution of the as-synthesized copolymer micelles were investigated by TEM and DLS. The ther- mosensitive behavior and micellization were examined with UV-vis and surface tension measurements. The experimental results showed that the as-synthesized P（NIPAM-co-NMAM）-g-（PLLA-g-MAAH） copolymers formed sphere-like micelles in aqueous solution, with an average size of approximate 20 nm, a low critical solution temperature of about 42 ℃, and a critical micelle concentration of ca. 28 mg/L. MTT assay indicated that the copolymers had no cytotoxicity or slight toxicity, and could be used as a potential biomedical material.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第5期116-121,125,共7页
Journal of Lanzhou University(Natural Sciences)
基金
国家自然科学基金资助项目(10675078)
关键词
接枝共聚物
聚N-异丙基丙烯酰胺
温敏性能
胶束化
细胞毒性
graft copolymers
poly（N-isopropylacrylamide）
thermosensitive property
micellization
cytotoxicity
