摘要
利用高速逆流色谱技术分离纯化金银花中的绿原酸。选择正丁醇-冰乙酸-水(4:1:5,V/V)系统来分离,分离结果经高效液相(HPLC)检测纯度达到98.1%,绿原酸的得率为95%。
High-speed countercurrent chromatography was used to separate chlorogenic acid from 70% ethanol extract of dry Lonicerajaponica Thunb. with three different solvent systems. It was found experimentally that the optimal solvent system is composed of butanol, acetic acid and wate, with the ratio of4：1：5 （V/V）. The purity of obtained chlorogenic acid is determined as 98.1% by high performance liquid chromatograph （HPLC）, and its yield is 95%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第11期298-299,共2页
Food Science
基金
陕西省重点实验室项目(O5JS12)
陕西理工学院科研基金项目(SLG0724)
关键词
绿原酸
高速逆流色谱
分离
chlorogenic acid
high-speed countercurrent chromatography （HSCCC）
seperation
