摘要
研究了用ＨＰＬＣ同时测定人血浆中氧氟沙星、诺氟沙星、洛美沙星和环丙沙星血药浓度的方法。采用ＳｐｈｅｒｉｓｏｒｂＣ（１８）色谱柱（２５０ｍｍ×４．６ｍｍ，５μｍ），以甲醇－０．００８ｍｏｌ／Ｌ磷酸盐缓冲液－０．５ｍｏｌ／Ｌ四丁基溴化铵（１６：７５：１．４，ｐＨ２．６）为流动相，检测波长２８０ｎｍ，血浆样品用乙腈沉淀蛋白后进样，简便快速，回收率稳定，对氧氟沙星，诺氟沙星，洛美沙星，环丙沙星四种氟喹诺酮药物检测的线性范围分别为：０．１０～５．００μｇ／ｍｌ；０．１１～５．４０μｇ／ｍｌ；０．１３～５．００μｇ／ｍｌ；０．１０～１０．００μｇ／ｍｌ。其方法检测限为：２０ｎｇ／ｍｌ；２０ｎｇ／ｍｌ；２５ｎｇ／ｍｌ及４０ｎｇ／ｍｌ，回收率均高于８６％。应用本法观察了健康人口服氧氟沙星和环丙沙星后的血药浓度变化。
An ion-pair reversed-phase high-performance, liquid chromatographic method was developod for the determination of four fluoroquinolones(ofloxacin,norfloxacin, lomefloxacin and ciprofloxacin)in human plasma. C(18) spherisorb column(250 mm×4.6 mm,5μm)and a mobile phase of methanol0. 008 mol/L phosphate buffer- 0.5 mol/L tetrabutylammonium bromide (16:75: 1.4,V/V/V,pH 2.6) were used. The detection wavelength was 280 nm. A simple single-step protein precipitation procedure was employed for pretreatment of plasma samples. The assay developed was found to be linear for ofloxacin(0.10～5.00μg/ml),norfloxacin(0.11～5.40μg/ml),lomefloxacin(0.13～5. 00μg/ml) and ciprofloxacin(0.10～10. 00μg/ml)with correlation coefficients>0.9994. The detection limits of the assay for ofloxacin, norfloxacin, lomefloxacin and ciprofloxacin were 20, 20,2 5 and 40 ng/ml, respectively.The recoveries of the drugs were over 8 6%. The method was employed for the determination of ofloxacin and ciprofloxacin in plasma samples from healthy volunteers following oral administration of a 200 mg ofloxacin tablet or a 400 mg ciprofloxacin tablet.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1994年第6期328-331,共4页
Journal of China Pharmaceutical University
关键词
氧氟沙星
诺氟沙星
洛美沙星
环丙沙星
血药浓度
HPLC
Ofloxacin
Norfloxacin
Lomefloxacin
Ciprofloxacin
Plasma concentration
