摘要
目的:建立连翘酯苷血药浓度的高效液相色谱测定方法,研究其在大鼠体内药代动力学变化规律。方法:大鼠静脉注射连翘酯苷50mg·kg^(-1)后,于不同时间点采血,用HPLC测定其血药浓度并用3p97软件拟合,计算药代动力学各参数。结果:连翘酯苷血药浓度的回归方程为Y=20598727 X-20590(r=0.9999),在1.95～250μg·mL^(-1)之间线性关系良好,最低检测浓度为0.8μg·mL^(-1)。静脉注射连翘酯苷50mg·kg^(-1)后,其体内过程经3p97软件拟合,药时曲线符合三室开放模型。结论:首次建立了连翘酯苷血药浓度的HPLC测定方法。方法操作简单、专属性强、灵敏度高。静脉注射连翘酯苷后,其体内过程的药时曲线符合三室开放模型,具有体内分布快、血药浓度下降迅速等特点。
Authors used the HPLC method to determine the forsythiaside in rats plasma, in an attempt to understand the pharmacokinetics of chrysophanol in rats after iv administration. In the study, rats were given forsythiaside by iv. administration. Blood samples were collected at different time. The forsythiaside concentration in plasma was determined using HPLC.The parameters of pharmacokinetics were calculated under a 3p97 program. A typical equation was estab lished to calculate the forsythiaside in rats/ plasma （Y=20598727 X-20590）. The calibration curve of forsythiaside was linear in the range from 1.95 to 250μg.mL^-1. （r=0.9999）.The lowest forsythiaside concentration was 0.2μg.mL^-1. After a single iv administration of forsythiaside in rats, the mean plasma concentration-time curve was found to fit three compartment models, and the main pharmacokinetics parameters were obtained. This is the first time to determine the concentration of forsythiaside in rats plasma using the HPLC method. The method has been proved highly sensitive and selective, desirable for studying the pharmacokinetics of forsythiaside. The forsythiaside concentrationtime curve is a three-compartment open model. The pharmacokinetics of forsythiaside shows a rapid absorption and quick elimination process in rats.
出处
《世界科学技术-中医药现代化》
2008年第4期53-56,100,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
山西省自然科学基金项目(2006011104):用于防治肺气肿的连翘活性成分的提取分离及作用机理，负责人：王进东。
