摘要
目的： 建立HPLC同时测定绿原酸、连翘酯苷A和黄芩苷的血药浓度，探析注射用双黄连粉针中3种成分在大鼠体内的药代动力学特征。方法： 采用HPLC同时测定绿原酸、连翘酯苷A和黄芩苷含量，以葛根素为内标，流动相乙腈（A）-0.1%磷酸（B）梯度洗脱（0~10 min，10%~20% A；10~35 min，20%~35% A；35~40 min，35% A；40~40.5 min，35%~10% A；40.5~55 min，10% A），绿原酸、连翘酯苷A、黄芩苷和葛根素的检测波长分别为328，328，277，249 nm。结果： 绿原酸和连翘酯苷A在0.2~20 mg·L-1、黄芩苷在2.0~300 mg·L-1线性关系良好（r〉0.999 4）；日内和日间精密度的RSD均〈13.6%，低、中、高3个质量浓度样品的方法回收率在88.3%-109.3%，提取回收率均〉80.8%。绿原酸、连翘酯苷A和黄芩苷的主要药动学参数AUC0-t分别为（8.406±1.175），（9.696±2.349），（145.35±22.02） mg·h·L-1；MRT0-t依次为（0.619±0.105），（0.634±0.115），（0.456±0.068） h；t1/2 Z分别为（0.532±0.064），（0.732±0.357），（0.542±0.175） h；Vz分别为（0.384±0.050），（0.673±0.422），（0.324±0.072） L·kg-1；CLZ分别为（0.504±0.067），（0.634±0.150），（0.426±0.066） L·h-1·kg-1。结论： 该方法灵敏、简便、准确，适用于大鼠血浆中绿原酸、连翘酯苷A和黄芩苷的测定及注射用双黄连粉针的药代动力学研究。
Objective： To develop a HPLC method for simultaneous determination of chlorogenic acid,forsythiaside A and baicalin in rat plasma,and investigate pharmacokinetics characteristics of these three ingredients from Shuanghuanglian powder injection. Method： Plasma samples were precipitated by acetonitrile-methanol （1：1）.HPLC was adopted to simultaneous determine contents of chlorogenic acid,forsythiaside A and baicalin with puerarin as internal standard,mobile phase composed of acetonitrile（A）-0.1% phosphoric acid（B） with a gradient elution program（0-10 min,10%-20% A;10-35 min,20%-35% A;35-40 min,35% A;40-40.5 min,35%-10% A;40.5-55 min,10% A）,detection wavelength of chlorogenic acid,forsythiaside A,baicalin and puerarin were 328,328,277,249 nm,respectively. Result： Excellent linear relationships were obtained in the range of 0.2-20 mg·L-1 for chlorogenic acid and forsythiaside A,but it was 2.0-300 mg·L-1 for baicalin （r〉0.999 4）;RSD of both intra-day and inter-day precisions were less than 13.6%,the assay recoveries at three levels were 88.3%-109.3%,the extract recoveries were more than 80.8%.Main pharmacokinetic parameters of chlorogenic acid,forsythiaside A and baicalin：AUC0-t were （8.406±1.175）,（9.696±2.349） and （145.35±22.02） mg·h·L-1;MRT0-t were （0.619±0.105）,（0.634±0.115） and （0.456±0.068） h;t1/2 Z were （0.532±0.064）,（0.732±0.357） and （0.542±0.175） h;Vz were （0.384±0.050）,（0.673±0.422） and （0.324±0.072） L·kg-1;CLZ were （0.504±0.067）,（0.634±0.150） and （0.426±0.066）L·h-1·kg-1,respectively. Conclusion： This determination method was sensitive,simple and accurate,it was suitable for determining contents of chlorogenic acid,forsythiaside A and baicalin in rat plasma and pharmacokinetics of Shuanghuanglian powder injection.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期168-171,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
四川省教育厅理科重点项目(12ZA026)
成都医学院创新实验项目(CX201224)
关键词
双黄连粉针
绿原酸
连翘酯苷A
黄芩苷
高效液相色谱法
血药浓度
药代动力学
Shuanghuanglian powder injection
chlorogenic acid
forsythiaside A
baicalin
HPLC
plasma concentration
pharmacokinetics
