摘要
从新疆天山一号冰川冻土中筛选到一株产低温碱性磷脂酶A1的菌株xjF1,初步鉴定为居泉沙雷氏菌(Serratia fonticola)。该菌属于耐冷菌,具有产两种酶的特性。其产生磷脂酶A1的最适碳源为木糖,最适氮源为磷酸氢二铵。在接种量为2%,250mL三角瓶装液量为50mL,25℃,250r/min摇床振荡培养36h时,磷脂酶A1活力最高达17.5U/mL。该磷脂酶A1的最适作用温度和pH值是35℃和9.0,在12℃仍具有69%的酶活力,但在60℃保温10min即丧失86%的活力,对热敏感。
A strain, x-jF1 producing mid-adapted alkaline phospholipase A1 was isolated from the No. 1 glacier soil samples of Mountain Turn, Xinjiang. On the basis of the observation of modality and the 16S rDNA sequence analysis, xjF1 was identified as Serratia fonticola. The strain belonged to psychrotroph and had the capability of producing two enzymes. Xylose was carlton source and （NH4）2HPO4 was nitrogen source for higher production of phospholipase A1. The strain produced maximum phospholipase A1 activity, as high as 17.5U/mL, after growth at medium volume 50mL in a 250mL flask, inoculation volume 2% at 2512 for 36h on a shaker with speed of 250r/min. The optimum temperature and pH for the phospholipase A1 activity were 35℃ and 9.0, respectively. The enzyme still remained 69% of its maximum activity at 12℃. However, it lost 86% of activity after incubation at 60℃ for 10min. The phospholipase A1 showed high thermolability.
出处
《工业微生物》
CAS
CSCD
北大核心
2008年第5期12-16,共5页
Industrial Microbiology
基金
新疆自然科学基金项目(编号:200421109)
新疆特殊环境微生物资源重点实验室项目(编号:XJYS0203-2004-06)
关键词
居泉沙雷氏菌
低温碱性磷脂酶A1
发酵条件
Serratia fonticola
cold-adapted alkaline phospholipase A1
fermentation conditions
