摘要
对罗汉果试管苗茎尖玻璃化法超低温保存进行了初步研究。结果表明，切取继代15d生长健壮的罗汉果试管苗茎尖，长2～3mm，在25℃下用60％PVS2（玻璃化保护液）预处理40min，再用100％PVS2在0℃处理50min，更换新鲜PVS2后投入液氮保存24h，于40℃水浴中快速解冻2min，用MS＋410．8g／L蔗糖的液体培养基洗涤40min，滤纸吸干后接种到MS＋1．0mg／L6-BA＋0．05mg／LNAA＋0．1mg／LGA3＋0．7％琼脂粉＋45g／L蔗糖的固体培养基上，25℃暗培养7d，转入正常光下培养。1周时的存活率最高可达94．56％。
The cryopreservation of the stem apex＇s of Fructus Momordicae in vitro by vitrification was preliminarily researched. The results showed that the stem apexes（2-3mm） from healthy in vitro subcultured 15 d were pretreated with 60% PVS2 for 40 min at 25℃ ,then treated with 100% PVS2 for 50 rain at 0℃ ,and then protected with fresh 100% PVS2 and put them into the liquid nitrogen. After 24h,these stem apexes were thawed rapidly in water for 2 min at 40℃ ,then rinsed in MS＋410.8 g/L sucrose for 40 min,after absorbing the liquid using filter paper,inoculated it on the medium MS＋1. 0mg/L 6--BA＋0.05 mg/L NAA＋0.1 mg/L GA3 ＋0.7% agar＋45 g/L sucrose,cultured in dark at 25℃ for 7 d,then transferred the stem apexes into the condition of normal lighting. So,the highest survival rate was up to 94.56% after 1 week.
出处
《河南农业科学》
CSCD
北大核心
2008年第10期102-105,共4页
Journal of Henan Agricultural Sciences
基金
广西壮族自治区自然科学基金资助项目(桂科自0542032)
广西师范大学博士启动基金资助项目
关键词
罗汉果
茎尖
玻璃化法
超低温保存
Fructus Momordicae
Stem apex
Vitrification
Cryopreservation
