摘要
A procedure on cryopreservation of shoot-tips of Eriobotrya japonica Lindl.had been studied in vitro by two steps vitrification.The effects of cold-hardening,preculture,cryoprotecten treatment,thawing and reculture on survival rate of the shoot-tips were tested.Optimal media for shoot-tip induction and propagation were 1/2 MS+1.0 mg·L-1 6-BA+0.5 mg·L-1NAA and MS+0.5 mg·L-16-BA+0.1 mg·L-1 NAA.Preculture for 30 min with 60% PVS2(30% glycerol+15% ethylene glycol+15% DMSO+0.4 mol·L-1 sucrose) and treatment for 50 min by PVS2 were suitable to shoot-tip cryopreservation.After cryopreservationsome shoot-tips couldregenerate plantlets,the regenerate rate was 46.8%.There was no difference in regenerating plantlets and tube plantlets not stored by cryopreservation.
A procedure on eryopreservation of shoot-tips of Eriobotrya japonica Lindl. had been studied in vitro by two steps vitrification. The effects of cold-hardening, preeuhure, eryoprotecten treatment, thawing and reculture on survival rate of the shoot-tips were tested. Optimal media for shoot-tip induction and propagation were 1/2 MS ＋ 1.0 mg · L^-1 6-BA ＋0.5 mg·L-1 NAA and MS＋0.5 mg·L-1 6-BA ＋0. 1 mg·L-1 NAA. Preculture for 30 min with 60% PVS2 （30% glycerol ＋ 15% ethylene glycol ＋ 15% DMSO ＋0.4 mol·L^-1 sucrose） and treatment for 50 min by PVS2 were suitable to shoot-tip eryopreservation. After cryopreservation some shoot-tips could regenerate plantlets, the regenerate rate was 46.8%. There was no difference in regenerating plantlets and tube plantlets not stored by eryopreservation.
出处
《植物资源与环境学报》
CAS
CSCD
2006年第2期75-76,共2页
Journal of Plant Resources and Environment
基金
国家自然科学基金资助项目(39970528)
关键词
枇杷
茎尖
超低温保存
玻璃化法
loquat （ Eriobotrya japonica Lindl. ）
shoot-tip
cryopreservation
vitrification
