摘要
对于香蕉这种营养繁殖的植物,超低温保存是长期有效地保存其种质的一种有效方法。主要综述了香蕉胚性悬浮细胞系、合子胚和茎尖分生组织的超低温保存技术方面近十几年来的研究进展。总结了影响香蕉超低温保存效果的几个主要因素,主要包括材料、预处理、装载处理以及冰冻保护剂。并分析了当前应用在香蕉分生组织上的4种超低温保存方法的优缺点,分别为:分生组织团简单冻存法、分生组织团玻璃化法、单个分生组织玻璃化法、滴冻玻璃化法,认为滴冻玻璃化法效果最好。
Since most banana （Musa spp.） do not produce seed and are vegetatively propagated, germplasm must be preserved clonally. Cryopreservation has been the most effective method for long-term conservation. Advances in the techniques for cryopreservation of Musa, including germplasm embryogenic cell suspension, zygotic embryos and apical meristems over ten years are reviewed ; and the main factors affecting cryopreservation are also mentioned, which include material, pretreatment, loading and dehydrating. By comparison of the four methods： simple freezing of proliferating meristems, vitrification of proliferating meristems, vitrification of isolated meristems, and droplet vitrification of isolated meristems for cryopreservation of meristems in banana, it is concluded that the droplet vitrification might be the best applicable protocol to Musa germplasm.
出处
《果树学报》
CAS
CSCD
北大核心
2005年第5期537-541,共5页
Journal of Fruit Science
基金
广东省攻关项目(编号2002A2080101102和C20209)
关键词
香蕉
种质
超低温保存
Banana（Musa spp.）
Germplasm
Cryopreservation
