摘要
目的观察甘草酸二铵(DG)对溃疡性结肠炎(UC)大鼠的治疗作用,并探讨其抗炎机制。方法40只雄性Wistar大鼠分为正常对照组,模型对照组,5-氨基水杨酸(5-ASA)组和DG组,每组10只。用2,4-二硝基氯苯+乙酸联合建模法制备UC大鼠模型。观察疾病活动指数(DAI)、结肠黏膜组织学变化及髓过氧化物酶(MPO)活性,放射免疫法检测血清白介素-6(IL-6)水平,免疫组织化学法检测核因子-κB(NF-κB)表达。结果与模型对照组相比,DG组和5-ASA组大鼠的DAI、组织学损伤评分、MPO活性、血清IL-6水平和结肠黏膜NFκ-B表达显著降低(P<0.05)。但DG组和5-ASA组各指标间差异无统计学意义(P>0.05)。结论DG可有效治疗UC,其作用机制可能是通过抑制NF-κB的活化及IL-6的产生和表达来抑制炎症反应,其疗效与5-ASA相当。
Objective To investigate the effects of diammonium glycyrrhizinate（DG）on ulcerative colitis（UC）in rats and to probe into its underlying mechanisms. Methods Forty male Wistar rats were randomly divided into 4 groups： control group, model gronp, 5-ASA group and DG group（ n - 10 in each group）. The rat colitis model was induced by 2,4-dinitrochlorobenzene（DNCB） and acetic acid. Disease activity index（DAI ）, colonic histology and myeloperoxidase（MPO） activity were observed. The expression of nuclear factor （ NF ）- κB in colonic mucosa was determined by immunohistochemistry. The level of interleukin-6 （IL-6） in serum was detected by radioimmunoassay. Results Compared with model group, DAI, lesions of colonic mucosa, MPO activity, serum level of interleukin- 6 and NF-κB expression in colonic mucosa in DG group and 5-ASA group decreased significantly（P 〈 0.05）, but there was no significant difference between DG group and 5-ASA group（P 〉0.05）. Conclusion The results indicate that DG could effectively treat UC by suppressing the activity of NF-κB and decreasing the expression of IL-6 in serum. DG has a similar effect as 5-ASA to reduce the inflammation of UC rats.
出处
《山西医科大学学报》
CAS
2008年第9期794-797,共4页
Journal of Shanxi Medical University
